摘要
MS is a chronic inflammatory disease of central nervous system in which T cells enter central nervous system and create an inflammatory cascade that leads to applying the other blood cells. Pre-inflammatory Cytokines are representative of the process of inflammatory diseases like MS. In addition, the increase of such cytokines has been observed in this kind of anomalies. On the other hand, helping T cells of 17 (TH17) contributes to the secretion of interleukin 17 that is a pre-inflammatory cytokine and an increase in TH17 cells is induced by interleukin 23 (IL23) which in turn causes absorption of neutrophils in the site of inflammation. Chemokine also has a determining function in the immune system including Ccl4 protein sit, the function of which is chemical absorption for calling natural fatal cells and monocytes as well as the other immune system cells;additionally, Ccl5 causes chemical absorption to absorb Eosinophil and Basophil and has an active role in applying Leukocytesin the site of inflammation. As a result, these 4 factors can hurt nervous cell through increasing its expression and calling leukocytes. The expression of these 4 genes, corticosteroid treatment and investigating its impact on changing the expression of such genes are the aims of this study. In this study 50 samples of blood of people suffer from multiple sclerosis (according to the diagnosis of specialist) (new case), 25 samples of blood of people suffer from MS (according to the diagnosis of specialist) using corticosteroid and 50 blood samples of healthy people without any symptoms were compared. To investigate the extent of expression of IL23, IL-17, CCL5 and CCL4 genes, first, the patients’ blood is taken, RNA was extracted and Real Time PCR was carried out. According to the results obtained from Real Time PCR of patients and healthy people it was specified that the amount of expression of chemokine and cytokines is increased in the people suffering from MS and has a direct relationship with MS. In comparing the two groups of pat
MS is a chronic inflammatory disease of central nervous system in which T cells enter central nervous system and create an inflammatory cascade that leads to applying the other blood cells. Pre-inflammatory Cytokines are representative of the process of inflammatory diseases like MS. In addition, the increase of such cytokines has been observed in this kind of anomalies. On the other hand, helping T cells of 17 (TH17) contributes to the secretion of interleukin 17 that is a pre-inflammatory cytokine and an increase in TH17 cells is induced by interleukin 23 (IL23) which in turn causes absorption of neutrophils in the site of inflammation. Chemokine also has a determining function in the immune system including Ccl4 protein sit, the function of which is chemical absorption for calling natural fatal cells and monocytes as well as the other immune system cells;additionally, Ccl5 causes chemical absorption to absorb Eosinophil and Basophil and has an active role in applying Leukocytesin the site of inflammation. As a result, these 4 factors can hurt nervous cell through increasing its expression and calling leukocytes. The expression of these 4 genes, corticosteroid treatment and investigating its impact on changing the expression of such genes are the aims of this study. In this study 50 samples of blood of people suffer from multiple sclerosis (according to the diagnosis of specialist) (new case), 25 samples of blood of people suffer from MS (according to the diagnosis of specialist) using corticosteroid and 50 blood samples of healthy people without any symptoms were compared. To investigate the extent of expression of IL23, IL-17, CCL5 and CCL4 genes, first, the patients’ blood is taken, RNA was extracted and Real Time PCR was carried out. According to the results obtained from Real Time PCR of patients and healthy people it was specified that the amount of expression of chemokine and cytokines is increased in the people suffering from MS and has a direct relationship with MS. In comparing the two groups of pat
作者
Rasam Hajiannasab
Rasam Hajiannasab(Medical Facility, Islamic Azad University of Najafabad, Najafabad, Iran)
