摘要
本文共进行三组实验,分别为起始时间为10~30 min、静水压压力为68.9 Mpa、持续时间为5 min的单因素实验一;起始时间为10~40 min、静水压压力为68.9 Mpa、持续时间为5 min的单因素实验二;以及起始时间(20, 30, 40 min)、压力大小(60, 65, 70 Mpa)、持续时间(3, 5, 7 min)的三因素正交实验三。实验一结果显示,当起始时间为30 min时,可以得到100%的三倍体率,并且该组的发眼率和上浮率相对于对照组均无显著性差异。实验二结果显示,综合发眼率、上浮率、三倍体率三个数据,最优的三倍体诱导起始时间为受精后的30~35 min。实验三结果显示,起始时间为20~40 min,静水压压力为60~70 Mpa,持续时间为3~7 min对三倍体率的影响无明显差异,在此时间范围内诱导都能够获得100%的三倍体率。结果说明,在保证子代一定的存活率的基础之上可以有效地获得100%的虹鳟三倍体。
In this study,three groups of experiments were carried out.Single factor experiment 1:starting time was 10~30 min,hydrostatic pressure was 68.9 Mpa,duration was 5 min;single factor expe-riment 2:starting time was 10~40 min,hydraulic pressure was 68.9 Mpa and the duration was 5 min;the three-factor orthogonal experiment 3:starting time(20,30,40 min),pressure(60,65,70 Mpa),duration(3,5,7 min).The results of experiment 1 showed that when the starting time was 30 min,100%triploid rate could be obtained,and the eyed rate and the floating rate of this group were not significantly different from those of the control group.The results of experiment 2 showed that the optimal induction time of triploid was 30~35 min after fertilization based on the data of eyed rate,floatation rate and triploid rate.The results of experiment 3 showed that there was no significant difference in the triploid rate in the experimental groups with a starting time of 20~40 min,a hydrostatic pressure of 60~70 Mpa and a duration of 3~7 min;within this time range,the induction rate of triploids can reach 100%.
出处
《水产研究》
2019年第2期53-62,共10页
Open Journal of Fisheries Research
基金
山东省农业良种工程项目(2016LZGC003)
山东省重点研发计划(产业关键技术)项目(2016CYJS04A01)资助。
