摘要
目的:观察氟、砷联合作用对体外培养人成骨细胞活性的影响,从而探讨氟、砷对骨的作用,砷是否对氟骨病有一定的影响。方法:体外培养人成骨细胞,氟对人成骨细胞的作用终浓度分别为10 mg/L、1 mg/L、0.1 mg/L、0 mg/L,砷对人成骨细胞的作用终浓度分别为1 mg/L、0.1 mg/L、0.01 mg/L、0 mg/L,相互交叉作用于人成骨细胞,观察细胞形态变化。结果:砷浓度为1 mg/L与各浓度氟联合作用时,各组细胞全部死亡。砷浓度为0.1 mg/L与各浓度氟联合作用于人成骨细胞,可见部分细胞死亡,细胞形态较完整,可见细胞核,周围少量细胞碎片。砷浓度为0.01 mg/L、砷浓度为0 mg/L与氟浓度为10 mg/L联合作用于人成骨细胞时,显微镜内可见少量细胞死亡及少许细胞碎片。其余联合实验组镜下可见较多人成骨细胞,轮廓清晰,细胞核完整,未见明显细胞碎片。结论:氟砷联合作用时,对人成骨细胞毒性一定程度的增加。
Objective: By observing the effect of fluorine and arsenic’s combination on activity of human os-teoblast in vitro, we defined the role they played in bone, and whether they influence bone meta-bolism or not. Method: Culture human osteoblast in vitro. When the concentration of fluorine reached 10 mg/L, 1 mg/L, 0.1 mg/L and 0 mg/L respectively, the concentration of arsenic reached 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0 mg/L, they mutually interact on human osteoblasts, and then we observe the change of cell morphology. Result: When arsenic concentration of 1 mg/L related with various concentration of fluorine mutual acted, all cells died;when arsenic concentration of 0.1 mg/L part of cells died, at the same time cell morphology was complete, the nucleus was visible, and there were some cell debris around. When arsenic concentration of 0.01 mg/L, 0 mg/L mutually acted with fluorine concentration of 10mg/L in human osteoblast, a few of cells died and a cellular debris can be seen in the microscope. For the osteoblast of other joint groups, the nucleus is complete without obvious cells fragments, and all cells outline is clear. Conclusion: The joint action of fluorine and arsenic could arouse a certain degree of increase in toxicity of osteoblast.
出处
《外科（汉斯）》
2018年第1期1-8,共8页
Hans Journal of Surgery
基金
贵州省科技厅社发攻关项目(NO:黔科合SY 字[2012]3125 号)
贵州省卫生厅基金项目(NO: gzwkj2012-1-023)。
