摘要
本文以油酸诱导HepG2细胞脂肪变性构建非酒精脂肪肝(Nonalcoholic fatty liver disease, NAFLD)体外细胞模型,通过测定细胞存活率、胞内TG含量、胞内脂滴油红O染色情况和脂质代谢相关基因mRNA表达研究荷叶生物碱提取物(Lotus leaf extract, LLE)对NAFLD脂质积聚的影响。结果显示,LLE对脂肪变性HepG2细胞的IC50为73.77 µg/mL,在无细胞毒性的浓度下,LLE可明显降低脂肪变性HepG2细胞中甘油三酯(Triglyceride, TG)含量和脂滴积聚程度,且高浓度作用更为明显。进一步研究发现,LLE对脂肪变性HepG2细胞中SREBP-1c和ACCα的mRNA表达影响不大,但能显著上调脂肪变性HepG2中PPARα、CPT-1和ACOX1的mRNA表达水平(p 5)。综上,LLE可抑制脂肪变性HepG2细胞增殖,降低细胞中TG含量和脂滴积聚,且该作用与TG的β-氧化分解有关。
In order to study the effect of lotusleaf extract (LLE) on liver fat accumulation induced by Nonalcoholic fattyliver disease, oleic acid-induced HepG2 cells was established and cellviability, TG contents and staining of lipid droplet and the expression oflipid metabolism related genes mRNA were detected. The results showed that the IC50 of LLE on oleic acid-induced HepG2 cells were73.77 µg/mL. LLE significantly reduced the content of TG and the lipid dropletsin oleic acid-induced HepG2 cells. LLE had little effect on the mRNA expressionof SREBP-1c and ACC, while it significantly increased the mRNA expression ofPPAR α, CPT-1 and ACOX1 in oleicacid-induced HepG2. It is concluded that LLE is effective on inhibiting on cellviability and to reduce lipid accumulation in oleic acid-induced HepG2 cells,which is more related to the up-regulation of lipid β-oxidative.
出处
《食品与营养科学》
2021年第2期72-79,共9页
Hans Journal of Food and Nutrition Science
