摘要
目的:研究非小细胞肺癌A549和H520细胞PDCD4和AP-1 (JunB和c-Fos)表达及其与细胞迁移的关系。方法:体外培养非小细胞肺癌肺腺癌细胞A549和肺鳞癌细胞H520,细胞划痕实验检测细胞迁移情况,western blot方法检测细胞PDCD4和AP-1 (JunB和c-Fos)表达情况,比较肺腺癌细胞A549和肺鳞癌细胞H520中PDCD4和AP-1 (JunB和c-Fos)表达差异以及细胞迁移能力的差异。结果:肺腺癌细胞A549中PDCD4蛋白表达明显低于肺鳞癌细胞H520,而AP-1 (JunB和c-Fos)蛋白表达明显高于肺鳞癌细胞H520,且肺腺癌细胞A549的迁移能力明显高于肺鳞癌细胞H520,差异有统计学意义(P < 0.05)。结论:肺腺癌细胞PDCD4蛋白表达低于肺鳞癌细胞,AP-1 (JunB和c-Fos)蛋白表达高于肺鳞癌细胞,细胞迁移能力与PDCD4表达呈负相关,与AP-1表达呈正相关。
Objective: To study the expression of PDCD4 and AP-1 (JunB and c-Fos) in non-small cell lung cancer A549 and H520 cells and their correlation with the migration ability of cells. Methods: Non- small cell lung cancer cells-lung adenocarcinoma cells A549 and lung squamous cell carcinoma cells H520 were cultured in vitro. Cell migration was detected by cell scratch test, and the expression of PDCD4 and AP-1 (JunB and c-Fos) was detected by Western blot. The expression difference of PDCD4 and AP-1 (JunB and c-Fos) and the difference of cell migration rate between lung adenocarcinoma cell A549 and lung squamous cell carcinoma cell H520 were compared. Results: The expression of PDCD4 protein in lung adenocarcinoma cell line A549 was significantly lower than that in lung squamous cell carcinoma cell line H520, while the expression of AP-1 (JunB and c-Fos) protein was significantly higher than that in lung squamous cell carcinoma cell line H520, and the migration ability of lung adenocarcinoma cell line A549 was significantly higher than that of lung squamous cell carcinoma cell line H520 (P < 0.05). Conclusions: The expression of PDCD4 protein in lung ade-nocarcinoma cells is lower than that in lung squamous cell carcinoma cells, and the expression of AP-1 (JunB and c-Fos) protein is higher than that in lung squamous cell carcinoma cells. The cell migration ability is negatively correlated with the expression of PDCD4 and positively correlated with the expression of AP-1.
出处
《临床医学进展》
2022年第7期6517-6524,共8页
Advances in Clinical Medicine
