摘要
以勃甜龙竹新萌发的嫩枝为外植体.初始培养基为3/4MS+6 BA2 0mg/mL+KT1 0+蔗糖20g/L(单位下同),丛生芽继代增殖培养基为3/4MS+6 BA2 0+NAA0 05mg/L(单位下同)+蔗糖20;生根培养基为1/2MS+NAA3 0+IBA5 0+蔗糖10,每一过程为20～30d,丛生芽20d继代1次,不定芽增殖3～5倍,生根约30d左右,生根率约为75%～80%,无菌苗移栽成活率90%以上.
We use new tender branches of dendrocalamas brandisii as the explant. The induction medica is3/4 MS supplemented with 2.0 mg/L BA,1.0 mg/L KT and 20 g/L sucrose.Medium for bud proliferaction is 3/4 MS containing 2.0 mg/L 6-BA,0.05 mg/L NAA and 20 g/L sucrose.Rooting culture medium is 1/2 MS with 3.0 mg/L NAA、5.0 mg/L IBA and 10 g/L sucrose.A regenerate period is 20 days.Buds proliferate coefficent is 3～5. 75～80 percent of bud are rooted.The survival rate is over 90 percent.
出处
《云南民族大学学报（自然科学版）》
CAS
2004年第3期203-206,共4页
Journal of Yunnan Minzu University:Natural Sciences Edition
关键词
勃氏甜龙竹
组织培养
生长调节剂
Dendrocalmus brandisii
tissue culture
the growing regulator
