摘要
目的 建立清脑宣窍方有效部位中藏红花酸的含量测定方法。方法 采用反相高效液相色谱 (RP HPLC)法测定藏红花酸含量 ,运用YWG C18色谱柱 (2 5 0mm× 4 6mm ,10 μm) ,流动相为甲醇-水 -冰乙酸 ,流速 :1mL min ,检测波长 :4 2 3nm。结果 藏红花酸在 0 0 2 2～ 0 110 μg范围内与其色谱峰面积呈良好的线性关系 ,r =0 9991,平均回收率为 99 4 % ,RSD =1 90 % (n =5 )。结论 本法操作简便、准确 ,可作为清脑宣窍方有效部位的质量控制方法之一。
Objective To establish a method for the quantitative determination of crocetin in the effective fraction of Qingnaoxuanqiao Formula (QF) by RP-HPLC. Method The RP-HPLC technique was used in the experiment. YWG-C 18 column (250 mm×4.6 mm, 10 μm) was used, the mobile phase was methanol-water-glacial acetic acid (83:17:34) at a flow speed of 1 mL/min, and the detection wavelength was 423 nm.Result Crocetin showed, between 0.022～0.110μg, a fine linear relationship with the peak areas (r=0.9991). The average recovery of this procedure was 99.4%, with RSD equal to 1.90% (n=5).Conclusion This procedure is easy and accurate, and can be used as one of the methods for controlling the quality of the effective fraction of QF.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2004年第3期63-65,共3页
Journal of Beijing University of Traditional Chinese Medicine
