摘要
依据HSV-1 Patton株糖蛋白D全基因序列,设计、合成一对引物,利用多聚酶链反应(PCR),从我国HSV-1 168株基因组DNA中扩增、克隆出糖蛋白D全基因序列,共1185bp,编码395个氨基酸,并将其推测的氨基酸序列同国外HSV-1 HFEM、SCl6、17和Patton株的糖蛋白D的氨基酸序列进行了详细比较,发现最保守的区域主要是除信号肽以外的胞外编码区。氨基酸的变异主要分布在信号肽区、跨膜区及胞浆内编码区。本工作对研究HSV-1糖蛋白D的结构和功能以及研制我国HSV-1基因工程疫苗都具重要意义。
According to the DNA sequence of glycoprotein D gene of HSV-1 strain Patton,two primers were designed and synthesized, a complete gly-coprotein D gene of HSV-1 strain 168 isolated in China was amplified thr-ough PCR reaction. The complete glycoprotein D gene sequence has 1185 nucleotides and encodes a polypeptide of 395 amino acids. Result obtained from the homology comparison among 168,HFEM,SC16,17 and Patton showed that the most consetved region was located in the ectodomain, whereas the most variable re靑ons in the signal peptide region,as well as transmembrane and cytoplasmic domain re靑ons. The present paper pro-vides the basis for further understanding of structure and function of HSV-1 gD gene and for a HSV vaccine development.
出处
《病毒学报》
CAS
CSCD
北大核心
1993年第4期308-315,共8页
Chinese Journal of Virology
基金
国家863高科技生物领域资助
关键词
单纯疱疹病毒
糖蛋白
聚合酶链反应
Herpes Simplex Virus-I, Glycoprotein D, Polymerase Chain Reaction
