摘要
目的 改良大鼠胃黏膜壁细胞分离方法。方法 采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。结果 台盼蓝染色 ,细胞存活率大于 95 % ;壁细胞纯度为 80 %～ 85 %。结论 这种分离方法简便易行 ,值得推广。
Objective To improve the means to isolate gastric parietal cells in rats.Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation.Results Trypan blue exclusion demonstrated >95 % viability. The purity of gastric parietal cell preparation was 80%～85%.Conclusion The improved method is simple and practicable, and worth of popularization.
出处
《徐州医学院学报》
CAS
2004年第3期242-243,共2页
Acta Academiae Medicinae Xuzhou
基金
江苏省教育厅自然科学研究计划资助项目 (0 2KJD310 0 0 8)
