摘要
目的 了解醋酸铅对人外周血淋巴细胞HPRT基因位点的影响 ,以探讨铅的致突变性。方法 采用多核细胞法体外研究醋酸铅对人外周血淋巴细胞HPRT基因位点的影响及突变频率。结果 0 2 5mmol/L醋酸铅即可诱发人外周血淋巴细胞HPRT基因位点突变 ,且突变频率随着染毒浓度的增加而升高 ,突变频率Y (1 0 -3 )与醋酸铅浓度C (mmol/L)之间存在一定的剂量 -效应关系 ,直线方程分别为 :Y =1 0 5 6 6 +0 1 6 6 1C (r=0 95 82 )和Y =1 0 76 2 +0 1 987C (r =0 94 34)。结论 在一定条件下 ,醋酸铅对人外周血淋巴细胞HPRT基因位点及突变频率有影响 。
Objective To study the mutational effect of PbAc 2 on HPRT gene of human peripheral lymphocytes in vitro.Methods A simple and sensitive method,cytokinesis block(CB) assay was used to detect the frequency of HPRT locus mutation in vitro induced by PbAc 2 and its dose effect relationship.Results The test showed that PbAc 2 could induce HPRT locus mutation in human lymphocyte and there existed a linear relationship between the concentration of the heavy metal saline and the mutation frequency(MF) of HPRT gene.Conclusions PbAc 2 could result in HPRT locus mutation in lymphocytes under this experimental conditions.It is expected that this assay might be used to study the environmental exposed population in future.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2004年第2期80-82,共3页
Industrial Health and Occupational Diseases
基金
江苏省自然科学基金社会发展项目 (BS2 0 0 10 5 0 )
关键词
醋酸铅
HPRT基因
突变频率
多核细胞法
PbAc 2
HPRT gene
Mutation frequency(MF)
Cytokinesis Block(CB) assay
