摘要
目的建立深部克柔念珠菌感染的豚鼠模型,并对其进行致病性研究。方法采用皮下注射曲安奈德和腹腔注射环磷酰胺诱导豚鼠进入免疫抑制状态后,静脉注射克柔念珠菌悬液,使豚鼠发生深部克柔念珠菌感染;与免疫正常组(对照组)豚鼠比较,观察两组死亡情况和脏器的组织载菌量,并进行统计学分析。结果免疫抑制组(实验组)豚鼠的死亡从感染后1d开始,3～4d达到高峰,至第6天止,其死亡率是正常组的10倍(P<0.01);各脏器的组织载菌量明显高于对照组(P<0.05);在检查的4种脏器中,最为易感的是肾脏,最不易感的是肝脏,各脏器间易感的差异有显著性(P<0.01)。结论本实验能有效诱导豚鼠的免疫抑制状态,明显增加豚鼠体内发生深部克柔念珠菌感染的概率;豚鼠的死亡率和组织载菌量是客观反映豚鼠体内真菌感染情况的敏感观察指标。用上述方法成功建立的深部克柔念珠菌感染豚鼠模型可用于后续抗真菌药体内抗真菌活性的研究。
Objective: To establish a guinea pig model for C. krusei infection caused by C.krusei and to study the pathogenicity of C.krusei on immunocompromising guinea pigs. Methods: immunosupressions in test group were induced by using cyclophosphamid and dexamethasone. The guinea pigs were infected with C. krusei suspension intravenously. Compared with immunocompetitive group(control group), the mortality and tissue burden of fungi were observed and analyzed by statistics. Results: The death of test group emerged on the 1st day after infection, peak at the 3rd and 4th day, then decreased gradually, finally ended at the 6th day. The mortality of test group was 10 times higher than that of control group (P < 0.01). Fungi tissue burdens of test group were much higher than that of control group (P < 0.05). The tissue burden of fungi was most hight in kidney, but lowest in liver, which was significant difference among those four observed organs (P < 0.01). Conclusions: Immunocompromise in guinea pigs, which can be induced effectively by using cyclophosphamid and dexamethasone, increased the incidence of invasive fungal infections remarkably. In this study, mortality and tissus burdern are sensitive markers reflecting the fungal infection in the guinea pigs. The guinea pig model with invasive fungal infection established by above described methods is successful, and can be used for further research.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2004年第6期331-333,共3页
Journal of Clinical Dermatology
关键词
动物模型
克柔念珠菌
深部感染
豚鼠
animal model
Candida krusei
deep infection
guinea pig
