摘要
寻找与喉癌发生进展的相关基因 ,应用比较基因组杂交技术分析了 18例喉癌患者。结果显示 ,每例喉癌细胞染色体均有不同程度的变化 ,包括染色体全部或部分的扩增或丢失。平均每例有 12 9处异常区域 ,丢失多于扩增 ,分别为每例 7 2处和 5 7处。主要为 3q(78% )、5 p(6 1% )、11q(5 6 % )、1q(5 0 % )、8p(4 4 % )、8q(39% )和 15q(39% )的扩增 ;3p(70 % )、5 q(78% )、9p(6 7% )、13q(5 0 % )、1p(4 4 % )和 14q(39% )的丢失。有多条染色体区带上出现特异的扩增或丢失 ,特别是 1p13 2 1(8/ 18)、3p2 1 2 3(14 / 18)、5 p2 1 2 2 (14 / 18)、9p12 pter(10 / 18)和 13q2 1 31(8/18)的拷贝数增加明显 ,而 1q11 2 1(11/ 18)、3q15 2 1(12 / 18)、8p2 2 2 4 (6 / 18)、11q12 13(8/ 18)、15q2 1 2 3(7/ 18)和18p11(8/ 18)区域的丢失为喉癌的特征性变化 ,说明这些区域中存在与喉癌发生发展密切相关的癌基因。
In order to find out the genes involved in the tumorigenesis of laryngeal carcinoma,we analyzed 18 laryngeal carcinoma with comparative genomic hybridization.Results show that each one has different degree of variances,included gains and losses of partial and whole chromosome.Each case has 12.9 abnormal regions averagely;losses are more than gains,(equal) to 7.2 and 5.7 per case respectively.Main regions are gains in chromosomes 3q(78%),5p(61%),11q(56%),1q(50%),8p(44%),8q(39%) and 15q(39%),and losses of 3p(70%),5q(78%),9p(67%),13q(50%),1p(44%) and 14q(39%).There are many specific gains and losses in several chromosomes,especially the increase of copy number karyotype in 1p13-21(8/18),3p21-23(14/18),5p21-22(14/18),9p12-pter(10/18)and 13q21-31(8/18),while the decrease in 1q11-21(11/18),3q15-21(12/18),8p22-24(6/18),11q12-13(8/18),15q21-23(7/18)和18p11(8/18) are the characteristic varieties.These results suggest that there are oncogene?tumor suppressor gene and other associated genes involved in the tumorigenesis.
基金
国家自然科学基金 (编号 :3 9770 794)项目资助~~
关键词
喉癌
比较基因组杂交
癌基因
抑癌基因
laryngeal carcinoma
comparative genomic hybridization
oncogene
tumor suppressor gene
