摘要
利用生物信息学手段对大肠杆菌和志贺氏菌的 1 1 0个O 抗原糖基转移酶与 39个O 抗原聚合酶的序列进行分析 ,探讨这两种酶的序列和结构特点。统计了其序列一致性 ,密码子使用和 (G +C) %含量的特点 ;讨论了O 抗原糖基转移酶和聚合酶对底物的特异性 ;推测了 6组糖基转移酶的功能 ;通过对蛋白拓扑结构的预测 ,发现O 抗原聚合酶中广泛存在一个位于细胞周质中的亲水环 (Loop) ,是可能的功能区域 ;通过对蛋白高级结构的预测 ,发现O 抗原糖基转移酶属于两个不同的蛋白超家族。
The sequential and structural characters of 110 glycosyltransferases and 39 polymerases (Wzy) of Escherichia coli and Shigella was studied using bioinformatic methods. The statistic data of sequence similarity, codon usage and (G+C)% content are shown. The substrate specificity of O-antigen glycosyltransferases and polymerases are proposed. Analysing amino acid sequences of all glycosyltansferases indicates fuctions of six different groups. It is found that a predicted periplasmic loop is present in every polymerase and is the putative functional region of polymerase. Based on their predicted 3D-structures, glycosyltransferases can be grouped into two protein super families.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第3期345-350,共6页
Acta Microbiologica Sinica
基金
国家"8 63计划" ( 2 0 0 2AA2Z2 0 5 1)
国家杰出青年科学基金资助项目 ( 3 0 12 5 0 0 1)~~
