摘要
为观察P2 7(Kip1)蛋白在常见白血病患者中的表达 ,探讨其与白血病类别、染色体异常、临床疗效和预后的关系 ,用蛋白印迹法加化学发光法对 82例初诊或复发白血病患者的骨髓或外周血进行P2 7(Kip1)蛋白的检测。结果显示 ,P2 7(Kip1)在急性淋巴细胞白血病 (ALL)中表达高于在急性非淋巴细胞白血病 (ANLL) (P =0 .0 33)和慢性粒细胞白血病 (CML) (P =0 .0 0 8)中表达。P2 7(Kip1)在慢性淋巴细胞白血病 (CLL)中表达高于在CML中表达 (P =0 .0 17)。P2 7(Kip1) >0 .6 5 5组首次化疗有效率 (CR和PR)高于P2 7(Kip1)≤ 0 .6 5 5组 ,两者有显著差异(P =0 .0 4 1)。急性白血病患者中 ,P2 7(Kip1) >0 .6 5 5组生存期长于P2 7(Kip1)≤ 0 .6 5 5组 ,P =0 .0 0 6 5 ;而且在ANLL(P =0 .0 2 71)、ALL(P =0 .0 2 6 6 )组中均有显著差异 ,P2 7(Kip1)表达高的 ,生存时间长 ,P2 7(Kip1)表达低的 ,生存时间短。P2 7(Kip1)表达低似乎与ALL患者多种染色体异常相关 (r =- 0 .775 ,P =0 0 4 )。而P2 7(Kip1)表达与白血病患者的性别、年龄、外周血白细胞数、原始细胞数、血LDH以及血尿酸水平无明显相关性。结论 :P2 7(Kip1)表达的高低与急性白血病的首次化疗效果、生存时间密切相关 ,低表达预示着不良预后。
To evaluate the expression of cyclin dependent kinase inhibitor P27(Kip1) in leukemia and to investigate its clinical significance,the P27(Kip1) protein in bone marrow or peripheral blood samples from 82 cases of leukemia was measured by Western blot and enhanced chemoluminenscence (ECL). The results showed that the expression of P27(Kip1) protein in ALL was higher than that in ANLL (P=0.033) and also that in CML (P=0.008). P27(Kip1) expression in CLL was higher than that in CML too (P=0.017).In acute leukemia,the effective rate (CR and PR) of initial chemical therapy in the group of P27(Kip1)>0.655 was higher than that in the group of P27(Kip1)≤0.655,P=0.041. For ANLL and ALL patients, the survival time in the group of P27(Kip1)>0.655 was longer than that in the group of P27(Kip1)≤0.655,P=0.0065. There were similar statistical significance for ANLL and ALL patients,P=0.0271 and P=0.0266 respectively. There was a negative correlation between chromosomal abnormalities and P27(Kip1) expression in ALL patients (r=-0.775,P=0.04). The expression of P27(Kip1) protein appeared nothing to do with sex,age,white blood cell number,blast cell number in peripheral blood,serum LDH or uric acid. In conclusion,the expression level of P27(Kip1) protein is in relation to the effect of initial chemical therapy and survival time,so that the lower P27(Kip1) expression may associated with poor prognosis in acute leukemia.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第3期291-297,共7页
Journal of Experimental Hematology
基金
南京医科大学科技发展基金资助项目 (编号NY0 1-61)
