摘要
目的 :研究间隙连接蛋白 4 3(Cx4 3)在子宫肌瘤中的表达及米非司酮作用后Cx4 3蛋白表达的改变 ,探讨子宫肌瘤的生长及治疗的相关机制。方法 :用免疫组化法检测 30例子宫肌瘤组织 ,2 0例术前 3d给予米非司酮的子宫肌瘤及 2 0例正常子宫肌组织中Cx4 3蛋白的表达。结果 :子宫肌瘤Cx4 3蛋白表达高于正常子宫肌组织 (P <0 .0 5 )。子宫肌瘤Cx4 3蛋白表达增生期高于分泌期 (P <0 .0 5 )。米非司酮作用后Cx4 3蛋白表达在增生期无明显改变 (P >0 .0 5 ) ,分泌期明显增高 (P <0 .0 5 )。结论 :子宫肌瘤中Cx4 3蛋白表达水平与月经周期相关 ,米非司酮对Cx4 3蛋白表达的上调可能是其治疗子宫肌瘤的机制之一。
Objective:To study the expression of connexin 43 protein in uterine leiomyoma and the changes of expression of connexin 43 protein in uterine leiomyoma after the treatment of mifepristone before operation,so as to explore the mechanism of development of uterine leiomyoma and mifepristone treatment on uterine leiomyoma.Methods:Normal myometrium tissues were obtained from 20 patients operated for other gynecologic diseases. Leiomyoma tissues were obtained from 30 uteri which were pathologically proved and 20 patients with uterine leiomyoma which administrating of mifepristone for 3 days before surgery. The expression level of connexin 43 protein of the samples were determined by immunohistochemisty and scored semiquantitatively.Results:(1) the expression level of connexin 43 protein in uterine leiomyoma was higher than that in normal myometrium (P<0.05).(2)Compared with those patients without being given mifepristone,the expression level of connexin 43 protein in the patients used mifepristone before operation was significantly increased in the secretory phase (P<0.05),and no difference in the proliferative phase (P>0.05).Conclusion:The level of expression of connexin 43 protein in uterine leiomyoma is relate to menstrual cycle.Mifepristone can treat uterine leiomyoma possibly through up-regulation of the expression of connexin 43 protein.
出处
《现代妇产科进展》
CSCD
2004年第3期204-206,共3页
Progress in Obstetrics and Gynecology
