摘要
利用 336个SSR标记和 10 0个RAPD引物 ,对分子标记辅助选择获得的 10个蜀恢 5 2 7抗水稻白叶枯病改良系的遗传背景、白叶枯病的抗性和抗谱及农艺性状进行了研究。结果表明 ,10个改良株系在Xa2 1和Xa4位点纯合 ,抗我国 7个病原型代表菌系CⅠ~CⅦ和菲律宾小种 1和 6 (P1和P6 )。在遗传背景分析检测的位点中 ,改良系与蜀恢 5 2 7表现差异的SSR位点仅为 1~ 5个 ,RAPD位点的相似率在 92 .1%~ 97.5 %之间。在考察的改良系及其与D6 2A和G4 6A所配制的杂交组合的 8个农艺性状中 ,除与G4 6A所配杂交组合在千粒重、每穗颖花数和产量性状方面与对照差异显著外 ,其余均无显著差异 ,但是所得改良系的抗性得到了提高 ,其余性状与轮回亲本相似 ,其中以Line 9和Line 10的遗传背景和农艺性状与蜀恢 5 2 7最为相似。表明分子标记改良蜀恢 5 2 7对白叶枯病的抗性是成功的 。
The genetic background, resistance spectrum to bacterial blight and agronomic traits were evaluated in 10 ‘Shuhui 527’ improved restoring lines with resistance to Bacterial blight, which were bred by Molecular Marker assisted Selection(MAS). Results showed that these lines were homozygous in loci Xa21 and Xa4 and resistant to the representative strains of 7 Chinese pathotypes CⅠ-CⅦ in China, and Philippine race 1 and 6, P1 and P6. Comparison of genetic background showed that there were only 1-5 differential SSR loci ,the similarity of RAPD bands was 92.1%-97.5%. The variation of the 8 agronomic traits observed between the improved restoring lines and combinations released from the each lines crossed with D62A was not significant .The agronomic traits investigated between the restoring line and the combinations of these lines crossed with G46A, respectively, had no significant difference except the 1 000 grain weight, spikelets per panicle and yield . But the resistance to Bacterial blight of these lines was improved and the other traits were similar to the recurrent parent. The genetic background and agronomic traits of line 9 and line 10 were more similar to Shuhui 527, it indicated that improvement of resistance to Bacterial blight of Shuhui 527 by using MAS was successful, and the MAS method used in rice breeding was a effectiveness approach.
出处
《作物学报》
CAS
CSCD
北大核心
2004年第6期563-567,共5页
Acta Agronomica Sinica
基金
国家 8 63计划项目 ( 2 0 0 1AA2 110 81)
教育部优秀博士论文资金 ( 2 0 0 0 5 4)
关键词
水稻
蜀恢527品种
白叶枯病
抗病改良系
遗传
农艺性状
Xa21 and Xa4
Genetic-background analysis
Bacterial blight resistance
Agronomic traits evaluation