摘要
应用细胞周期流式细胞仪(FCM)分析与~3H—TdR掺入法药敏测定技术,对8种化疗药物(MMC,MTX,5—Fu,ADM,CDDP,VP_(16),VCR,DTIC)在29例肝癌体外培养细胞的抑制作用与细胞周期比例的关系进行了对比研究。结果显示,化疗药物抑制率与细胞周期比例有关。以抑制率>60％作为有效标准,则对8种化疗药物有效的22例肝癌(75.9％)的细胞周期比例明显不同于抑制率<60％的肝癌。我们认为,FCM细胞周期测定具有简便、快速、客观、准确的特点,在临床个体化疗上具有实际应用价值。
The relationship between tumor cell phases analysed by flow cytome-try (FCM) arid inhibition rates of eight chemotherapy drugs (MMC, MTX, 5-Fu, ADM, CDDP, VP16, VCR, and DTIC) detected by 3H-TdR incorporation in twenty-nine heptocellular carcinoma (HCC) was studied. The results showed that there was a significant correlation between tumor cell phases and inhibition rates of chemotherapy drugs. The tumor cell phases ratio of twenty-two HCC (75.9%) with>60% inhibition rates were significantly different from seven HCC with <60% inhibition rates. We consider that the method of cell-phase analysis by FCM is simple, quick and precise, and is valuable in routine use of chemotherapy invidnalization.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1993年第3期207-209,共3页
Chinese Journal of Cancer
关键词
肝肿瘤
流式细胞计
药敏试验
Liver neoplasm Flow cytometry Drug sensitivity testing in vitro
