摘要
本文应用黑腹果蝇(Drosophila melanogaster)性连锁隐性致死(Sex Linked Recessive Lethal,SLRL)试验检测了氯化汞(HgCl_2)对雄性果蝇生殖细胞的致突变性,结果表明:1—2d龄的Oregon-K雄蝇用0.1％HgCl_2喂饲染毒24h后,其子2代的突变率为0.9％。0.2％HgCl_2喂饲染毒的突变率为1.38％。高、低剂量组的突变率均超过自然突变率(0.26％)2倍余,并呈剂量一效应关系,用Kastenbaum-Bowmon(KB)检验方法进行统计学分析,试验组与阴性对照组的突变率差异非常显著(P<0.01)。说明氯化汞可诱发雄性果蝇生殖细胞染色体基因发生突变,即具有致突变作用。但氯化汞对果蝇生殖细胞不同发育阶段(精母细胞、精细胞、成熟精子)所致突变率差异无显著性。
The effect of mercuric chloride (HgCl2) on mutagenicity in male germ cell of Drosophila melanogaster was studied by Sex Linked Recessive Lethal (SLRL) test. The results showed that the mutation frequencies of F2 generation of 1-2 day old Oregon-K male fed with 0.1% and 0.2% HgCl2 for 24 hours were 0.9% and 1.38% respectively. The mutation frequencies of both high and low dosage groups were twice of more higher than spontaneus one (0.26%) and a dose effect relation was revealed between them. Statistically significant difference was found between treated and control groups by Kastenbaum-Bowman test. It is indicated that mercuric chloride can induce gene mutation of sex chromosome in male Drosophila. There was no statistical significance in the difference of mutation frequencies among three stages of germ cell development.
出处
《癌变．畸变．突变》
CAS
CSCD
1993年第5期17-19,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
氯化汞
黑腹果蝇
生殖细胞
致突变
mercuric chloride
Drosophila melanogaster
, Sex Linked Recessive Lethal
mutagenicity
