摘要
用表达型噬菌体载体ZAP构建了忽地笑叶片cDNA文库。文库宿主菌为E.coliXL1 BLUE,亚克隆空菌为E.coliXLOLR。初始文库的滴度为5.6×105pfu/mL,扩增文库的滴度为6.9×109pfu/mL,含插入片段的频率为96%,插入片段大小在0.5～2.5kb,文库质量良好。为进一步从基因组学和分子生物学方面研究忽地笑叶片发育及克隆相关全长基因奠定了基础。
Lycoris aurea is a perennial,bulbiferous plant.Its vegetative growth and reproduction are discrete.To study the leaf development of Lycoris aurea,A cDNA library was constructed from Lycoris aurea leaves.The titer of primary library is 5.6×10~5 pfu/mL,and the titer of amplified library is 6.9×10~9 pfu/mL,in which 96% of the phages were recombinant.Insert sizes ranging from 500 bp to 2 500 bp were obtained.All of the above mentioned accorded with the general requirements of cDNA library construction,which established a good foundation for investigating the leaf development and cloning full-length cDNA of genes related to leaf development of Lycoris aurea with the methodology of both genomics and molecular biology.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第3期86-88,共3页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家"863"项目(2002AA241051)
