摘要
对从我国河南某地野鼠体内分离的狂犬病病毒野毒株MRV糖蛋白基因进行反转录-PCR,分别得到了1067 bp和938 bp 2个cDNA片段,对PCR所得片段进行序列测定并推导出相应的氨基酸序列。采用计算机软件分析了该毒株G基因的4个功能区中核苷酸和氨基酸的变异特点,在3个抗原位点上,与同一基因型中其他毒株相比,只有个别位点的变异。比较了该毒株和其他所选比较毒株之间的变异特点,G基因抗原区与CVS株的同源性可以达到98％,与ERA株的同源性达90％。讨论了G基因中与毒性相关的核苷酸和氨基酸的特点。
Glycoprotein gene(about 1 067 bp and 938 bp) of rabies virus strain from wild mouse(MRV) , isolated from mouse somewhere in Henan province of China, was amplified by reverse transcription -polymerase chain reation (RT- PCR) . The variant characteristics of nucleotide and amino acid in the 4 functional sections of this G-gene were analyzed by computer with DNAStar software. MRV of three an-tigetic sites was compared with that of the other strains and analyzed in the same genotype. Only few sites varied. Meanwhile, characteristics of nucleotide sequence and amino acid of the site in the G-gene and toxicity related were analyzed too. Homologies of nucleotide and amino acid of MRV with CVS and ERA were 98.0% and 90% respectively. The characteristics of nucleotide sequence and amino acid of the points in the G-gene and toxicity related were discussed.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2004年第2期210-212,216,共4页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30070542)
