摘要
目的 评价胰岛素对培养的牛胸主动脉内皮细胞血管内皮生长因子 (VEGF)受体flt 1、flk 1 KDR表达的影响及其与一氧化氮合酶 (NOS)的关系。方法 取新生的小牛胸主动脉 ,作血管内皮细胞原代及传代培养 ,取 4 6代培养细胞用于实验 ;应用不同浓度 ( 30mU L、30 0mU L、30 0 0mU L)的胰岛素和NOS抑制剂 (L NAME)干预培养过程 ,4 8h后取培养细胞 ,应用免疫组化法测定flt 1、flk 1 KDR和NOS3的表达水平。结果 不同浓度胰岛素孵育组后内皮细胞flt 1、flk 1 KDR的表达水平差异无显著性 ,L NAME孵育后各组内皮细胞flt 1、flk 1 KDR的表达水平较单纯胰岛素孵育组差异无显著性。结论 胰岛素对内皮细胞flt 1、flk 1 KDR的表达无直接影响 ;内皮细胞NOS3的活性不是内皮细胞VEGF受体flt 1、flk 1
Objective To investigate the expression of VEGF receptor (flt 1? flk 1/KDR) protein by cultured bovine thoracic aortic endothelial cells at various insulin concentrations and its relation to the NOS 3 activation of endothelium Methods Endothelial cells were separated from bovine thoracic aorta, and cultured in serum free medium, then incubated at various insulin concentrations (30 mU/L?300 mU/L?3?000 mU/L) and the NOS inhibitor L NAME (2 mmol/L) The levels of VEGF receptor (flt 1?flk 1/KDR) and NOS 3 protein were detected by use of immunohistochemical staining Results The expression of VEGF receptor (flt 1?flk 1/KDR) protein in bovine thoracic aortic endothelial cells had no significant difference ( P >0 05) between the insulin incubation groups both with and without L NAME incubations Conclusion Insulin has no direct effect on the expression of VEGF receptor (flt 1? flk 1/KDR) protein in bovine thoracic aortic endothelial cells The NOS 3 activation of endothelium is not the main cause that could affect the expression of VEGF receptor (flt 1?flk 1/KDR) protein of endothelium
出处
《中国介入心脏病学杂志》
2004年第2期111-114,共4页
Chinese Journal of Interventional Cardiology
