摘要
目的 通过重组 c DNA表达文库的血清学分析 (SEREX)技术获得来自大鼠睾丸文库有潜在治疗价值的异种同源抗原的 c DNA序列。方法 将人卵巢癌细胞株免疫兔后收集的抗血清作为筛选血清 ,采用 SEREX技术筛选大鼠睾丸 c DNA文库。结果 通过两轮筛选获得 10个阳性克隆。通过生物信息学分析认为这 10个阳性单克隆 c DNA序列编码 7种不同的蛋白 ,其中 OV- 2、OV- 4为肿瘤相关序列 ,OV- 6、OV- 7为编码未知蛋白的序列。结论 用异种免疫血清筛选异种同源肿瘤抗原拓宽了 SEREX技术的应用范围 ,是一种行之有效的方法。
Objective It is the intent of this study to analyzed the rat testis library by employing a modified SEREX (serological analysis of recombinant cDNA expression library) approach to find the xenogeneic homologous tumor antigens which could be useful in developing cancer vaccines. Methods The screening serum was obtained from the immunized rabbits with human ovarian cancer cells, and 10 positive clones were isolated from the rat testis cDNA library using SEREX technology. Results We found these 10 clones encoded seven different proteins by means of bioinformatic analysis. Among them, OV-2 and OV-4 encode proteins related to carcinoma in human, yet OV-6 and OV-7 are novel and their encoded proteins remain unknown. Conclusion This study indicates that the utilization of xenogeneic immunized serum in the serological screening of xenogeneic homologous tumor antigens may expand the application of traditional SEREX technology.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第3期305-308,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家 8 63计划 (资助号 2 0 0 2 AA2 2 2 0 3 1)资助
