摘要
目的 从大鼠氧自由基代谢水平探讨微波对急性胰腺炎大鼠模型的治疗作用。方法 将 3 0只SD大鼠随机分为 3组 ,即假手术组、模型组及实验组。假手术组、模型组术后均无特殊处理。实验组大鼠分别于造模后 3h、9h及 2 3h行上腹部微波辐射治疗。各组大鼠均于术后 2 4h经内眦采血检测血清淀粉酶(AMY)、超氧化物歧化酶 (SOD)及丙二醛 (MDA)的含量变化并同时观察胰腺组织病理学改变。结果 实验组大鼠血清淀粉酶含量升高幅度低于模型组 (P <0 .0 5 ) ;实验组血清SOD含量明显高于模型组 (P <0 .0 1) ;实验组血清MDA含量明显低于模型组 (P <0 .0 5 ) ;光镜观察发现实验组大鼠胰腺病理改变程度明显轻于模型组。结论 急性胰腺炎大鼠存在脂质过氧化损伤 ,光镜下可见其胰腺组织发生严重病理改变。微波治疗可降低急性胰腺炎大鼠模型的AMY ,MDA含量 ,提高SOD含量 ,减轻胰腺组织病理改变 。
Objective To investigate the curative effect of microwave on metabolism of oxygen free radicals in rats with acute pancreatitis. MethodsThirty rats were recruited and divided randomly into three groups: sham operation group(SHAM), sample model group (SAP)and microwave therapy group(MWT). The rats in MWT group were treated with microwave diathermy at 3h,9h,23h after the pancreatitis model was established. The concentration of serum amylase(AMY), superoxide dismutase(SOD) and malondialdehyde(MDA) were measured and the pathological changes of pancreatic tissue were observed at 24h after operation in all rats. ResultsAs compared with the SAP group, the concentration of AMY in rats in the MWT group was significantly lower ( P <0.05); SOD significantly higher; and MDA significantly lower ( P <0.05). The pathohistologic alterations of the pancreatic tissues in rats of the MWT group were significantly alleviated in comparison with that of the SAP group. ConclusionIt was indicated that there existed lipid peroxidation-induced injury in rats with acute pancreatitis. Microwave diathermy inhibited production of oxygen free radical in rats with acute pancreatitis.As a result, we could infer that microwave diathermy had the curative effect on acute pancreatitis.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2004年第2期77-80,共4页
Chinese Journal of Physical Medicine and Rehabilitation
