摘要
目的 建立小鼠溃疡性结肠炎 (UC)动物模型 ;应用双歧杆菌活制剂 ,观察其对UC发生、发展的影响 ,并检测其对TNF α、IL 1β的表达和NF κB活性的影响。方法 用 5%葡聚糖硫酸钠(DSS)口饲法制备小鼠UC模型 ;给小鼠口服双歧杆菌 (Bifidobacterium ,Bf)活菌 ,观察UC的症状和组织学变化 ;采用逆转录 聚合酶链反应 (RT PCR)技术 ,检测UC小鼠肠黏膜内TNF α、IL 1β的表达水平进行半定量测定 ;应用免疫电泳迁移率改变分析 (electrophoreticmobilityshiftassay ,EMSA)法检测UC小鼠肠黏膜细胞NF κB的激活。结果 应用双歧杆菌制剂后 ,UC的症状、组织损害均较模型组明显减轻。同时TNF α、IL 1β的表达较单纯模型组降低 (P <0 .0 5) ,NF κB的核结合活性也降低 ,但较正常对照组仍明显增加。结论 生态制剂Bf对UC的发生有预防作用 ,可能是通过抑制NF κB的核结合活性 。
Objective To investigate the effects of Bifido bacterium on the expression of cytokines and activation of nuclear factor κB in murine intestinal mucosa with ulcerative colitis(UC). Methods UC was induced in mice with oral inoculation of DSS for seven days. The expression of TNF-α, IL-1β in the intestinal mucosa of mice were detected by a semi-quantitative assay, RT-PCR. The activation of NF-κB in the intestina l mucosa were evaluated by electrophoretic mobility shift assay (EMSA). The rele vance of oral inoculation of Bifidobacterium and the expression of cytokines and the activation of NF-κB were analyzed. Results Com paring to the mice treated with DSS alone, both symptoms and the lesions of colo nic mucosa were slighter in the animals orally inoculated with Bifidobacterium during the induction of colitis than that of control. Furthermore, the expres sions of TNF-α, IL-1β were significantly decreased(P<0.05). The activ ation of NF-κB was also suppressed. Conclusion Bifido bacterium had both prophylaxis and therapeutic effects on UC, that was probabl y due to the suppression of the activity of NF-κB and the subsequent inhibiton of the expression of proinflammatory cytokines.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2004年第1期40-43,共4页
Chinese Journal of Microbiology and Immunology
