摘要
目的 :本研究用基因表达谱芯片研究桃仁蛋白A(PSPA)对小鼠纤维肉瘤基因表达的影响。方法 :将 4 0 96条小鼠cDNA用点样仪点在特制玻片上制备成表达谱芯片 ,利用实验组和对照组肿瘤组织的mRNA通过逆转录方法 ,将Cy3和Cy5两种荧光分别标记到两种组织的cDNA上 ,制成cDNA探针 ,并与表达谱芯片进行杂交及扫描 ,重复 2次实验。 结果 :通过计算机数据处理 ,筛出差异表达的基因 6 4条。结论
AIM: To study the effect of peach seed (semen Persicae) on gene expression of mouse with sarcoma 180 by means of DNA microarray. METHODS: DNA microarrays were made by spotting PCR products of 4096 mice cDNA noto a specially treated glass slides. The probes were prepared by labeling experimental and control group tumor tissue mRNA with Cy3 dUTP and Cy5 dUTP separately through reverse transcription. The arrays were then hybridized against the cDNA probe mixture and the fluorescent signals were scanned. The data obtained were analyzed from two repeated experiments. RESULTS: 64 genes were obtained by computer processing. CONCLUSION: These genes identified through this approach are considered as potential candidates for PSPA anti tumor effect.
出处
《中成药》
CAS
CSCD
北大核心
2004年第5期398-401,共4页
Chinese Traditional Patent Medicine
基金
哈尔滨铁路局科委科研经费资助项目 (No .2 0 0 1 0 0 9)
关键词
蛋白A桃仁
抗肿瘤
基因芯片
protein A, Semen Persicae
antitumor effect
DNA microarray
