摘要
目的 建立α粒子辐射诱导人肺鳞癌裸鼠转移模型。方法 α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种裸鼠皮下 ,后采用组织块接种的方法 ,在鼠间连续传代 3次 ,计算原代成瘤率及传代成活率 ,并观察肿瘤的转移情况 ,病理切片和免疫组织化学鉴定肿瘤的性质和来源。结果 (1)α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种成瘤率为 4 4 4 % (4 9) ;(2 )原代肿瘤的体表转移率与肺转移率均为 2 5 % (1 4 ) ;(3)病理切片鉴定为低分化鳞状上皮细胞癌 ;(4)免疫组织化学显示CK3和EMA表达阳性。结论 采用组织块接种的方法 ,在α粒子辐射诱导人肺鳞癌细胞系BERP35T4的基础上 ,经过裸鼠体内连续传代 3次 ,成功地建立了人类肺癌裸鼠转移模型。
Objective To establish a metastatic model of human lung carcinoma induced by α particles in nude mice. Methods A total of 9 BALB/C nude mice (5-6 weeks old) was inoculated subcutaneously (SC) with human tumor cell line BERP35T4.The tumor nodules developed from the nude mice were passaged for 3 times in nude mice via orthotopic implantation of histologically intact tissues. Results (1)Four of 9 nude mice developed (44 ^4%)neoplasm at the initial inoculation site.(2)one of four nude mice with neoplasm (25%) presented subcutaneous and lung metastasis.(3)Tumor tissue isolated from nude mice was characterized as squamous epithelial carcinoma with low differentiation.(4)CK3 and EMA staining were positive in cancer cells. Conclusion {A metastatic } model of human lung carcinoma induced by α particles in nude mice has been established via orthotopic implantation of histologically intact tissues. ;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2004年第1期16-18,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家重点基础研究专项资金资助项目 (G19980 5 12 0 7)
