摘要
目的 探讨不同电泳方法在淋巴瘤T细胞受体γ(TCR γ)基因重排研究中的应用及其意义。方法 在聚合酶链式反应 (PCR )中采用T细胞受体γ基因重排通用引物 ,对 3 0例T细胞淋巴瘤和 3 0例B细胞淋巴瘤组织扩增 ,并采用琼脂糖凝胶电泳和单链构型多态性分析实验对扩增产物进行研究。结果 2 9例T细胞淋巴瘤扩增产物在琼脂糖电泳中为阳性 ,其中 2 7例在SSCP分析实验中为阳性。 16例B细胞淋巴瘤在琼脂糖电泳中为阳性 ,其中 3例在SSCP分析实验中阳性。所有在琼脂糖电泳中阴性的PCR产物在SSCP分析实验中也为阴性。在琼脂糖电泳中真阳性与假阳性条带有差异。结论 在TCR γ基因重排扩增产物分析中 ,琼脂糖凝胶电泳可能产生假阳性结果 ,但无假阴性 ;琼脂糖凝胶电泳中的假阳性可在SSCP实验中排除。 2种电泳方法同时应用 ,可以减少工作量 。
Objective To explore the application and significance of different methods of electrophoresis in study about lymphoma T cell receptorγ(TCR-γ) gene rearrangement.Methods Consensus primers for T cell receptorγgene rearrangement were adopted in polymerase chain reaction.Tissues of 30 cases of T cell lymphomas and 30 cases of B cell lymphomas were amplified,and the amplification products were researched by agarose gel electrophoresis and single-stranded conformational polymorphism(SSCP) analysis.Results The amplification products of 29 cases of T cell lymphoma were positive in agarose gel electrophoresis and 27 among the 29 cases had positive results in SSCP analysis.The amplification products of 16 cases of B cell lymphoma were positive in agarose gel electrophoresis and 3 of the 16 cases had positive results in SSCP analysis.All those cases with negative results in agarose gel electrophoresis also had negative results in SSCP analysis.There were some difference between the true positive bands and false positive bands in agarose gel electrophoresis.Conclusion In analysis of amplification productions of TCR-γgene rearrangement,agarose gel electrophoresis may produce false positive result,but no false negative result.The false positive result in agarose gel electrophoresis can be excluded by SSCP analysis.Application of both the two means can reduce the amount of work and improve the veracity.
出处
《实用癌症杂志》
2004年第2期113-116,共4页
The Practical Journal of Cancer
基金
广东省科技计划项目 (B30 1 0 1 )
关键词
淋巴瘤
T细胞受体Γ
基因重排
聚合酶链式反应
琼脂糖凝胶电泳
单链构型多态性
Lymphoma
T cell receptor γ(TCR-γ)
Gene rearrangement
Polymerase chain reaction(PCR)
Agarose gel electrophoresis
Single-stranded conformational polymorphism(SSCP)
