摘要
在甲基磺酸溶剂中,间苯二酚和偏苯三甲酸酐反应,得到6-和5-羧基荧光素异构体的混合物,反应产物与特戊酰氯,二异丙胺反应,分离得到带有二特戊酰基保护的6-羧基荧光素二异丙胺盐,水解后酯化,得到6-羧基荧光素琥珀酰亚胺酯。
Synthesis of active 6-carboxyfluorescein succinimidyl esters was described. Resorcinol was condensed with trimellitic acid in methylsulfonic acid as a solvent to give a mixture of 5-(6-)carboxyfluorescein isomers. The obtained carboxyfluorescein was then reacted with pivalyl chloride and di-isopropy lamine, the purified 6-carboxyfluorescein dipivalate di-isopropy lamine salt was separated. After hydrolysis, tne product of 6-carboxyfluorescein dipivalate di-isopropylamine salt was to be esterized to give the active ester.
出处
《染料与染色》
CAS
2004年第2期93-94,共2页
Dyestuffs and Coloration
基金
国家自然科学基金资助项(20376010)
关键词
荧光素
异构体
特戊酰氯
二异丙胺
水解
fluorescein active ester
pivalyl chloride
di-isopropylamine
