摘要
目的 探讨多发性肌炎 (PM)患者外周血淋巴细胞免疫表型的变化及其与临床的关系。方法 采用流式细胞仪测定 2 3例PM患者和 18例健康者CD3+、CD4 +、CD8+、CD2 8+、CD8+/CD2 8+、CD4 5RA +、CD4 +/CD4 5RA +、CD4 +/CD2 9+、CD19+、CD5 +/CD19+、CD5 6 +、HLA DR +、CD3+/HLA DR +、CD6 9+百分率。结果 PM患者淋巴细胞CD8+和CD8+/CD2 8+百分率均低于健康者 (P <0 .0 5 ) ,CD3+/HLA DR +百分率高于健康者 (P <0 .0 5 ) ;CD3+、CD4 +、CD2 8+、CD4 5RA +、CD4 +/CD4 5RA +、CD4 +/CD2 9+、CD19+、CD5 +/CD19+、CD5 6 +、HLA DR +、CD6 9+百分率与健康者的差异无显著性 ;病程≤ 2个月的PM患者CD8+和CD8+/CD2 8+百分率低于病程 >2个月者 ;不同免疫表型的表达水平与临床和实验室检查无关 (P >0 .0 5 )。结论 CD8+淋巴细胞 ,尤其是CD8+/CD2 8+淋巴细胞在PM的发病机制中起作用。
Objective To investigate the patterns of peripheral blood lymphocytes immunolophenotype in polymyositis(PM) and the correlations with clinical findings. Methods The percentage of CD3+, CD4+, CD8+, CD28+, CD8+/CD28+, CD45RA+, CD4+/CD45RA+, CD4+/CD29+, CD19+, CD5+/CD19+, CD56+, HLA-DR+, CD3+/HLA-DR+, CD69+ were measured by flow cytometry from 23 PM patients and 18 age, sex matched healthy controls. Results Compared with the controls, the percentages of CD8+ and CD8+/CD28+ lymphocytes were decreased, the percentage of CD3+/HLA-DR+ lymphocytes were elevated in patients with PM(P<0.05) while CD3+, CD4+, CD28+, CD45RA+, CD4+/CD45RA+, CD4+/CD29+, CD19+, CD5+/CD19+, CD56+, HLA-DR+, CD69+ were not significantly different from those of the controls. The percentages of CD8+ and CD8+/CD28+ in the patients with disease course ≤ 2 months were lower than those>2 months. There were no significant correlations between the levels of immunolophenotype and clinical, laboratory findings(P>0.05). Conclusion CD8+ lymphocytes, especially the CD8+/CD28+ lymphocytes play a role in the pathogenesis of polymyositis.
出处
《上海医学》
CAS
CSCD
北大核心
2004年第4期246-249,共4页
Shanghai Medical Journal
关键词
多发性肌炎
外周血淋巴细胞
免疫表型
检测
流式细胞仪
Polymyositis
Flow cytometry
Immunophenotyping
Antigen, CD8
Human leucocyte antigen-DR
