摘要
目的 探讨干红葡萄酒 (DRW )在细胞、分子、基因调控水平的抗动脉粥样硬化 (AS)作用 ,从而为DRW防治AS提供实验佐证。方法 采用病理技术、电泳迁移率改变分析法 (EMSA)和原位杂交术检测 4 5只食饵性AS新西兰兔 4周、8周和 12周分别处死 ,观察血管壁的病理变化、核转录因子 (NF κB)活化、单核细胞趋化蛋白 (MCP 1)与蛋白激酶 (PKCα)mRNA表达情况及DRW对其干预的影响。结果 与食饵性AS兔组各相应时相点相比干红葡萄酒可显著抑制不同阶段AS血管组织血管壁的增生、血管组织细胞NF κB的活化 (4周 :18 5± 0 6和 13 7± 0 3;8周 :2 6± 0 9和17 8± 0 5 ;12周 :39 9± 1 2和 2 7 8± 0 8) ,并显著下调其MCP 1、PKCαmRNA的表达 ,且具有时间依赖性 ,DRW干预 12周时作用最强。结论 提示DRW可能是通过抑制NF κB活性 ,减少MCP 1、PKCαmRNA表达 ,从而阻抑了AS组织的损伤 。
Objective To study the effects of dry red wine in the different stages of experimental atherosclerosis (AS) at the cell, molecular and gene regulation levels in order to provide scientific basis for using dry red wine in the prevention of atherosclerosis Methods Blood vessel wall pathological changes, activity of NF k B and the expressions of monocyte chemotactic protein 1 (MCP 1) and protein kinase C (PKC α) were observed in dietary induced atherosclerosis rabbit model by morphology study, electrophoretic mobility shift assay (EMSA), and in situ hybridyzation, and the effects of dry red wine intervention were examined Results Dry red wine significantly suppressed the prolifreration of atherosclerosis intima and NF κB activation(4w:18 5±0 6 vs 13 7±0 3;8w:26±0 9 vs 17 8±0 5;12w:39 9±1 2 vs 27 8±0 8),and down regulated the expressions of MCP 1 and PKC α Conclusions The results confirmed that dry red wine could protect AS tissues and prolong its development by suppressing NF κ B activation, down regulating the expressions of MCP 1 and PKC α,which may take part in pathogenesis of AS
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2004年第2期103-106,F004,共5页
Chinese Journal of Preventive Medicine
