摘要
目的 探讨喉鳞癌p27基因表达水平及其临床意义。方法 用原位杂交方法检测49例喉鳞癌、19例声带息肉、12例癌旁增生组织及8例非典型增生组织中p27的表达。结果 喉鳞癌组织p27阳性表达率12.24％(6/49),声带息肉63.16％(12/19),癌旁增生组织33.33％(4/12),非典型增生组织25.00％(2/8),喉鳞癌组织p27mRNA表达显著低于其他各组(P<0.05),与临床分期有显著相关性(P<0.05)。结论 喉鳞癌组织中存在p27基因的低表达,与喉癌临床分期显著相关。
Objective To investigate the expression of P27 gene in the tissues of laryngeal carcinoma and its clinical implications. Methods Included in this study were 49 samples of laryngeal squamous cell carcinoma(LSCC) , 19 samples of vocal cord polyp, 12 samples of paracancer-ous tissue and 8 samples of atypical hyperplasia tissue. The expression of P27 mRNA in these samples were determined by in situhybridization procedures. Results The positive rate of P27 mRNA expression was 12.24% ( 6149 ) in LSCC, 63. 16% ( 12/19 ) in the polyp of vocal cord, 33.33%(4/12)in the paracancerous tissues,and 25.00(2/8)in the atypical hyperplasia tissues, with a significant correslation of p27 expression with the clinical staging of LSCC (P<0.05). Conclusions The expression of p27 is down-regulated in LSCC and its expression is closely related with the staging of this lesion.
出处
《中国中西医结合耳鼻咽喉科杂志》
2004年第2期60-62,共3页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
关键词
P27基因
喉鳞癌
基因表达
肿瘤
原位杂交
p27 gene
Laryngeal squamous cell carcinoma
In situhybridization
