摘要
目的 研究亚硒酸钠对氯化镉诱导的大鼠肝细胞DNA损伤、细胞凋亡、细胞增殖周期变化以及DNA相对含量的影响。方法 选用雄性SD大鼠 ,每组 5只 ,用 5 μmol/kg亚硒酸钠分别与5、10和 2 0 μmol/kg的氯化镉联合作用 ,腹腔注射染毒。用单细胞凝胶电泳研究DNA损伤 ,用末端标记法 (TUNEL)和流式细胞术检测大鼠肝细胞凋亡 ,用流式细胞术研究细胞增殖周期和DNA相对含量。结果 5 μmol/kg的亚硒酸钠对 5、10和 2 0 μmol/kg的氯化镉引起的DNA损伤和细胞凋亡显示出良好的拮抗作用 ,也可使DNA损伤率和细胞凋亡率显著下降。 5 μmol/kg的亚硒酸钠明显抑制5 μmol/kg氯化镉引起的G0 /G1期细胞减少 ,并使 10 μmol/kg和 2 0 μmol/kg氯化镉引起减少的G2 /M期细胞显著增多。 5 μmol/kg的亚硒酸钠还对 10 μmol/kg和 2 0 μmol/kg氯化镉引起的DNA相对含量的下降表现出拮抗作用。结论 一定剂量的亚硒酸钠对一定剂量的氯化镉诱导的DNA损伤、细胞凋亡。
Objective This study was conducted to study the effects of sodium selenite on rat hepatocellular DNA damage, apoptosis, changes of cell cycle and DNA relative content induced by cadmium chloride in vivo Methods Both sodium selenite at the dose of 5 μmol/kg and cadmium chloride at the dose of 5 μmol/kg, 10 μmol/kg and 20 μmol/kg were given to rats by i p and there were 5 male SD rats in each group Hepatocellular DNA damage was measured by the single cell gel electrophoresis (or comet assay), hepatocellular apoptosis was measured with TUNEL (TdT mediated dUTP Nick End Labelling) and flow cytometry, DNA relative content (DNA RC ) and cell cycle were detected with flow cytometry Results When sodium selenite at the dose of 5 μmol/kg acted jointly with cadmium chloride at the dose of 5 μmol/kg, 10 μmol/kg and 20 μmol/kg respectively, the results showed that selenium reduced the effect of cadmium on DNA damage and apoptosis and decreased the rates of DNA damage and the rates of apoptosis significantly Sodium selenite at the dose of 5 μmol/kg increased cell number of G 0/G 1 period decreased by cadmium chloride at the dose of 5 μmol/kg and increased cell number of G 2/M period decreased by cadmium chloride at the dose of 10 μmol/kg and 20 μmol/kg Sodium selenite at the dose of 5 μmol/kg increased DNA relative content reduced by cadmium chloride at the dose of 10 μmol/kg and 20 μmol/kg Conclusions It was suggested that selenium at certain doses could antagonize DNA damage, apoptosis, changes of cell cycle and DNA relative content induced by cadmium in rat hepatocytes in vivo
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2004年第3期155-158,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助 (3 0 2 71110 )
