摘要
目的建立一种ELISA方法 ,检测患者血清中抗转铁蛋白抗体 ,评价其对子宫内膜异位症诊断的价值。方法用转铁蛋白抗原包被 ,间接ELISA方法检测患者血清中抗转铁蛋白抗体。采集患者血清 ,分 3组 :盆腔内异症组 38例 ,Ⅰ、Ⅱ期 2 0例 ,Ⅲ期 9例 ,Ⅳ期 9例 ;妇科良性疾病对照组 30例 ;正常对照组 4 0例。结果内异症患者血清中抗转铁蛋白抗体阳性率高达 94 .7% ,正常对照组阳性率为 2 .5 % ,良性疾病对照组阳性率为 0 % ,内异症组与两对照组比较差异有显著意义 (P均 <0 .0 1 )。间接ELISA法诊断内异症临床敏感性为 94 .7% ,特异性为 98.6 % ,诊断符合率为 96 .5 %。内异症各组阳性率比较差异无显著意义 (P >0 .0 5 )。结论用转铁蛋白抗原包被的间接ELISA法检测血清中抗转铁蛋白抗体诊断内异症敏感性、特异性强 ,诊断符合率高 。
Objective To establish ELISA method for the detection antibody to transferrin in the serum of patients with endometriosis (EMS) and to investigate its clinical value for diagnosing endometriosis. Methods An ELISA method was established by using transferrin as coating antigen to detect the serum samples from 38 female with endometriosis (20 cases with stage Ⅰ Ⅱ, 9 cases with stage Ⅲ, 9 cases with stage Ⅳ), 30 benign lesions, 40 normal female control. Results The positive rates of antibody transferrin to in the serum of patients with endometriosis (specificity 98.9%, sensitivity 94.7% and accuracy 97.6%) were significantly higher than those in control group ( P <0.01). There were no defferences among stages of endometriosis. Conclusion Detecting antibody transferrin to may will provide specific noninvasive diagnosis for endometriosis.
出处
《贵州医药》
CAS
2004年第5期406-407,共2页
Guizhou Medical Journal
