摘要
AlM: To study the expression of neurokinin-1 receptor (NK-1R) and neurokinin-2 receptor (NK-2R) in distal ileum ofacute necrotizing pancreatitis (ANP) and to evaluate therelationship between expression of these two receptors andintestinal mucosal damage.METHODS: A total of 130 adult Sprague-Dawley rats wererandomly divided into two groups: the rats in ANP group(n＝80) were induced by the retrograde intraductal infusionof 30 g@L-1 sodium taurocholate. And the rats in normal controlgroup (n＝50) received laparotomy only. Sacrifices weremade 6 h, 12 h, 24 h and 48 h later in ANP and normalcontrol group after induction respectively. Intestinal mucosalpermeability was studied by intrajejunal injection of 1.SmCiradioactive isotope 99mTc-diethlene triamine pentacetic acid(DTPA) and the radioactivity of 99mTc-DTPA content in urinewas measured 6 h, 12 h, 24 h and 48 h after induction.Then the pancreas and intestine were prepared forpathology. Reverse transcription polymerase chain reaction(RT-PCR) was used to determine the mRNA expression ofNK-1R and NK-2R, and Western blot was used to investigatethe protein level of NK-1R and NK-2R.RESULTS: In ANP rats, serious histologic damages inintestinal mucosa were observed, and the radioactivity of99mTc-DTPA in urine increased significantly in the ANP group.RT-PCR revealed that NK-1R and NK-2R mRNA level wasoverexpressed in the distal ileum of ANP as compared withthe normal control group. Western blot discovered strongerNK-1R (14-fold increase) and NK-2R (9-fold increase)immunoreactivity in the intestinal mucosa of ANP rats.Moreover, the overexpression of NK-1R was associated withmucosal pathological score (r＝0.77,P＜0.01) and intestinalpermeability (r＝0.68, P＜0.01) in ANP rats.CONCLUSION: NK-1R and NK-2R contribute to disruptedneuropeptides loop balance, deteriorate intestinal damage,and are involved in pathophysiological changes in ANP.
AIM:To study the expression of neurokinin-1 receptor(NK- 1R)and neurokinin-2 receptor(NK-2R)in distal ileum of acute necrotizing pancreatitis(ANP)and to evaluate the relationship between expression of these two receptors and intestinal mucosal damage. METHODS:A total of 130 adult Sprague-Dawley rats were randomly divided into two groups:the rats in ANP group (n=80)were induced by the retrograde intraductal infusion of 30 g·L^(-1)sodium taurocholate.And the rats in normal control group(n=50)received laparotomy only.Sacrifices were made 6 h,12 h,24 h and 48 h later in ANP and normal control group after induction respectively.Intestinal mucosal permeability was studied by intrajejunal injection of 1.5mCi radioactive isotope ^(99m)Tc-diethlene triamine pentacetic acid (DTPA)and the radioactivity of ^(99m)Tc-DTPA content in urine was measured 6 h,12 h,24 h and 48 h after induction. Then the pancreas and intestine were prepared for pathology.Reverse transcription polymerase chain reaction (RT-PCR)was used to determine the mRNA expression of NK-1R and NK-2R,and Western blot was used to investigate the protein level of NK-1R and NK-2R. RESULTS:In ANP rats,serious histologic damages in intestinal mucosa were observed,and the radioactivity of ^(99m)Tc-DTPA in urine increased significantly in the ANP group. RT-PCR revealed that NK-1R and NK-2R mRNA level was overexpressed in the distal ileum of ANP as compared with the normal control group.Western blot discovered stronger NK-1R(14-fold increase)and NK-2R(9-fold increase) immunoreactivity in the intestinal mucosa of ANP rats. Moreover,the overexpression of NK-1R was associated with mucosal pathological score(r=0.77,P<0.01)and intestinal permeability(r=0.68,P<0.01)in ANP rats. CONCLUSION:NK-1R and NK-2R contribute to disrupted neuropeptides loop balance,deteriorate intestinal damage, and are involved in pathophysiological changes in ANP.
基金
the scientific research funding for the returned overseas Chinese scholars,State Personnel Ministry,No.7690004027
