摘要
目的 研究人端粒酶逆转录酶 (hTERT)在外阴白斑和外阴鳞癌中的表达 ,探讨其在外阴白斑和外阴鳞癌发生、发展中的意义。方法 采用原位杂交法检测 80例外阴白斑、3 3例外阴鳞癌和 2 3例正常皮肤组织中hTERT的表达。结果 hTERT在外阴白斑各分型中阳性率分别为 :增生型 3 6.3 6% ,硬化萎缩型 4.17% ,混合型 2 3 .81% ,不典型增生型 46.15% ;在正常皮肤组织中阳性率为 13 .0 4% ;在外阴鳞癌中阳性率为 87.88% ,与外阴白斑和正常组织表达率相比差异有非常显著性 (P <0 .0 1)。hTERT在Ⅰ级、Ⅱ级、Ⅲ级外阴鳞癌中阳性表达率分别为 84.62 % (11/ 13 )、88.89%(8/ 9) ,90 .91% (10 / 11) ;鳞癌各级之间比较差异无显著性 (P >0 .0 5)。结论 原位杂交检测hTERT能够间接反映端粒酶活性 ,hTERT的异常表达可能与外阴白斑和外阴鳞癌的发生。
ObjectiveTo investigate the expression of hum an telomerase reverse transcriptase (hTERT) and to evaluate its clinical signifi cance in the occurrence,development of genitalia leukoplakia and squamous cell c arcinoma of vulva.MethodsUsing the method of hybridization in situ,human t elomerase reverse transcriptase (hTERT) were detected in 80 cases of genitalia l eukoplakia,33 cases of squamous cell carcinoma of vulva and 23 cases of normal s kin.ResultsThe positive rates of hTERT in different types of genitalia leukoplakia were 36.36% in hyperplasia type,4.17% in sclerotic type,2 3.81% in mixed type,46.15% in atypical hyperplasia and 13.04% in normal skin res pectively.The positive rate of hTERT was 87.88% in patients with squamous cell c arcinoma of vulva and was significantly higher than that of types of genitalia l eukoplakia as well as normal skin (P<0.01).The positive rates of hTERT in th e different grades of squamous cell carcinoma of vulva were 84.62%(11/13) in gra de Ⅰ,88.89%(8/9) in grade Ⅱ and 90.91%(10/11) in grade Ⅲ (P>0.05) res pectively.ConclusionThe results suggested that expressio n of hTERT might indirectly reflect the telomerase activity and the aberrant exp ression of hTERT was closely related to the occurrence,development of genitalia leukoplakia and the tumorgenesis and progression of squamous cell carcinoma of vulva.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2003年第5期297-300,共4页
The Chinese Journal of Dermatovenereology
关键词
端粒酶逆转录酶
外阴白斑
外阴鳞癌
原位杂交法
检测
Genitalia leukoplakia
Squamous cell carcinom a of vulva
Telomerase reverse transcriptase
Hybridization in situ
