摘要
研究了长寿花胚性愈伤组织的筛选,胚状体的诱导发生、发育过程及植株再生。经过对外观及细胞学观察,看出外观质地疏松、颗粒状的淡黄色愈伤组织细胞圆形且形状规则,是胚性愈伤组织。诱导胚性愈伤组织的最适培养基为:MS+2,4-D 2 mg·L-1+BA 0.2 mg·L-1;胚状体的诱导培养基为MS+BA 2 mg·L-1+NAA 0.2 mg·L-1+活性炭4 g·L-1+蔗糖30 g·L-1,胚状体诱导率可达185个·g-1胚状体;胚状体的再生培养基为MS+蔗糖30 g·L-1。利用石蜡切片对胚状体的发生过程进行了观察。
The study aimed on the selection of embryonic callus of Kalanchoe blossbeldiana, the induction of embryoid, morphogenesis of embryoid and regeneration. The grain-like, light yellow and loose callus was embryonic callus. During the inducing of embryoid, the culture mediums with different grouth regulator concentration were used. Finally the culture medium with the highest of reduction frequency was MS + BA 2 mg · L-1 + NAA 0. 2 mg · L-1 + Sucrose 30 g · L-1 + Active carbon 4 g · L (185 embryoid per gram). The regeneration medium was MS + Sucrose 30 g · L-1. The appearance and growing of embryoid were also observed through paraffin slices of callus. The development procedure of embryoid was procell of embryoid, globular stage, heart-shape stage and cotyledonary stage.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第2期249-252,共4页
Acta Horticulturae Sinica
