摘要
目的 探讨P27和cyclinE蛋白在人骨肉瘤中的表达及其在骨肉瘤发生、发展中的作用。方法 采用免疫组化S-P法检测30例骨肉瘤和10例骨软骨瘤中P27和cyclinE蛋白的表达。结果 在骨肉瘤和骨软骨瘤两组中的P27蛋白阳性表达率分别为33.3％和80.0％,两者差异显著(P<0.05)。而且,两组中P27蛋白表达强度的差异有显著性(P<0.05)。在骨肉瘤和骨软骨瘤两组中的cyclinE蛋白阳性表达率分别为93.3％和30.0％,两者差异显著(P<0.05)。两组中cyclinE蛋白表达强度的差异有显著性(P<0.05)。在骨肉瘤中P27和cyclinE蛋白的表达强度呈显著负相关(r_s=-0.432,P<0.05)。结论 P27蛋白在骨肉瘤中低表达,cyclinE蛋白在骨肉瘤中高表达,两者有相关性,均与骨肉瘤发生、发展有关。
Objective To evaluate the expression of P27 and cyclinE proteins in carcinogenesis and development of osteosarcoma. Methods S - P immunohistochemical method was used to detect the expression of P27 and cyclinE proteins in 30 cases of osteosarcoma and 10 cases of osteochondroma. Results The positive rate of P27 protein was 33. 33% in osteosarcoma and 80% in osteochondroma, and there was significant difference(P < 0. 05 ) between them. Moreover, there was significant difference (P <0. 05) in staining intensity of P27 protein between two groups. The positive rate of cyclinE protein was 93. 33% in osteosarcoma and 30% in osteochondroma, there was significant difference( P < 0. 05 ) between them. There was significant difference ( P < 0. 05 ) in staining intensity of cyclinE protein between two groups. There was negative correlation in staining intensity between the expression of P27 and cyclinE proteins in osteosarcoma. ( rs = -0. 432, P < 0. 05 ). Conclusion The expression of P27 is lower and cyclinE is higher in osteosarcoma. It is suggested that P27 and cyclinE proteins may play an important role in carcinogenesis and development of osteosarcoma.
出处
《中国骨肿瘤骨病》
CAS
2004年第2期125-128,共4页
Chinse Journal Of Bone Tumor And Bone Disease
