摘要
目的 :研究乳杆菌 (Lactobacillusacidophilussp )O 2菌株生长和积累类细菌素所需的营养和温度条件。方法 :采用分段控温发酵法 ,即在发酵后期 ,改变温度继续发酵 ;以发酵液抑菌圈直径为指标对发酵的营养和温度条件进行筛选。结果 :后期 32℃控温发酵有利于类细菌素积累 ,优化后的 4号配方 ,发酵液菌数高达 8 2× 10 10 个 /ml(细菌计数器法 ) ,抑菌圈直径最大 ,达到 8 8mm。结论 :利用 4号配方进行分段控温发酵能有效地促进O 2菌株的生长繁殖和积累类细菌素。
Objective:To study the optimal cultural media and temperature of L acidophilus sp O 2 strain for synthesizing bacteriocin like substance.Methods:Fermentation by stage method was adopted.Inhibition zones of fermented broth were used to select the superior cultural media and more suitable temperature in the fermentation of L acidophilus sp O 2 Results:Controlling the temperature at 32 ℃ after 18 h is proper for synthesizing bacteriocin like subestance.The total count of L acidophilus sp O 2 in fermented broth was up to 8 2×10 10 /ml in media and its inhibition zone was 8 8 mm Conclusion:4# medium and stage method,which can enhance the bacteriocin like substance synthesized in the broth,are effective reproduction factors of L acidophilus sp O 2
出处
《中国微生态学杂志》
CAS
CSCD
2004年第1期15-17,共3页
Chinese Journal of Microecology
