摘要
本文报道采用高效表达载体PBS(tac启动子)在大肠杆菌(E·coli)中表达乙型肝炎病毒核心抗原(HBcAg)的方法.重组质粒在异丙基一硫代半乳糖苷(IPTG)的诱导下,其表达效率提高近1000倍;经溶菌酶—SDS破壁、DNase处理后,离心上清液中含有大量HBcAg.通过对IPTG诱导浓度、诱导时间及细菌起始诱导浓度等因素的研究.我们找到一系列高效表达HB-cAg的条件,表达滴度高达1:25600。
A method which uses high-level expression vector PBS (tae promoter) to express the HBV core antigen gone has been reported. Under the induction of iso-propylthio-β-D-galactosidc(IPTG), the expression level of rccombinant plasmid h-as been increased 1000 times. After treatment with lysozyme-SDS-DNase system, the supernatant of crude bacterial lysale includes a large a mount of HBcAg.Through the studies of I1PTG induction concentration,IPTG induction time and the bacterial stale of initial induction, we have found a series of conditions to express the HBcAg gene efficiently. The highest liter of HBcAg is 1:25600
出处
《重庆医科大学学报》
CAS
CSCD
1992年第2期81-85,共5页
Journal of Chongqing Medical University
关键词
乙型肝炎
核心抗原
基因表达
Hepatilis B Virus
Core Antigen. Gene Expression
