摘要
目的 构建原核分泌型表达载体并高效表达胰岛素样生长因子I(IGF I)。方法 构建出 2种分泌型表达载体 ,命名为pCSA和pCST ,并将胰岛素样生长因子I(IGF I)基因插入pCSA和pCST中 ,转化E .coliW3110 ,经筛选获得工程菌pCSA/IGF I/W3110和pCST/IGF 1/W3110 ,并进行表达。结果 经SDS PAGE检测 ,在相对分子质量 76 0 0处有明显表达带 ,Westernblot表明重组蛋白具有IGF I的抗原性。结论 原核分泌型IGF I的高效表达 ,为进一步研究人IGF
Objective To construct a prokaryotic vector for secretory expression of human insulin like growth factor I(hIGF I).Methods Construct 2 secretory expression vectors pCSA and pCST,then insert hIGF I into the 2 vectors and transform to E.coli W3110 respectively.Select recombinant strains pCSA/IGF I/W3110 and pCST/IGF W3110 for expression.Results SDS PAGE profile showed a clear protein band with a relative molecular weight of 7 600.Western blot proved that the recombinant protein had the antigenicity of IGF I.Conclusion hIGF I was successfully expressed in prokaryotic cells.It laid a foundation of further study on the function and biological properties of hIGF I.
出处
《中国生物制品学杂志》
CAS
CSCD
2004年第1期1-3,共3页
Chinese Journal of Biologicals
基金
吉林省科技发展计划资助
吉科合字第 19980 5 77
