摘要
目的 探讨抗CD4 4单克隆抗体A3D8对HL 6 0细胞增殖及分化的影响 ,寻找急性髓系白血病(AML)治疗的新靶点。方法 以AML细胞株HL 6 0为模型 ,通过观察细胞生长、形态学、表面分化抗原、细胞周期和细胞因子表达 ,以及应用硝基四氮唑蓝 (NBT)还原实验来研究抗CD4 4单克隆抗体A3D8对细胞增殖及分化的影响。结果 HL 6 0细胞高表达CD4 4 ;A3D8以浓度和时间依赖性方式抑制HL 6 0细胞生长。A3D8使HL 6 0细胞周期阻滞在G0 /G1期 ,CD11b、CD14表面抗原阳性率明显增高 ,细胞体积增大 ,核 /浆比例减小 ,核染色质聚集 ,NBT还原实验阴性 ,细胞表达粒细胞集落刺激因子mRNA ,呈现向单核细胞系方向分化。结论 A3D8可抑制HL 6 0细胞增殖 ,并诱导其向单核系细胞分化 ,CD4 4有可能成为AML治疗的新途径。
Objective To appraise the effects of A3D8, an anti-CD44 monoclonal antibody on proliferation and differentiation of HL-60 cells ,so as to find out whether CD44 can be used as a therapeutic target in AML.Methods Through observations on cell growth, morphology, surface differentiation antigen, cell cycle, cytokines expression, NBT reduction and expression of G-CSF mRNA; outcome with to study of the influences of A3D8 on proliferation and differentiation of HL-60 cell line.Results A3D8 inhibited the proliferation of HL-60 cells in a dose- and time-dependent manner with blockage of cell growth at the G0/G1-phase in a concentration of 2.0 μg/ml and increased the expression of the surface markers, CD11b and CD14. In addition, the cells treated with A3D8 demonstrated a trend of differentiation toward monocytic lineage with expression of G-CSF mRNA.Conclusions A3D8 can inhibit the proliferation of HL-60 and induce the cells to differentiate along monocyte-macrophage lineage and CD44 may serve as a novel potential therapeutic target for AML.
出处
《上海医学》
CAS
CSCD
北大核心
2004年第3期190-192,共3页
Shanghai Medical Journal
基金
国家教委留学人员科技活动项目择优资助
