摘要
目的 探讨核内不均一核糖核蛋白A2 /B1(hnRNPA2 /B1)在非小细胞肺癌 (NSCLC)中的表达特征及其临床意义。方法 应用免疫组织化学S P法检测hnRNPA2 /B1在 5 8例NSCLC及癌旁组织、支气管切缘和 3 0例肺良性病变肺组织中的表达 ,并分析该蛋白表达与肺癌临床病理特征的关系。结果 NSCLC组织中hnRNPA2 /B1阳性表达率 ( 63 .79% )显著高于癌旁肺组织 ( 4 3 .10 % )和肺良性病变肺组织 ( 2 0 .0 0 % )(P =0 .0 0 0 ) ,癌旁肺组织中hnRNPA2 /B1阳性率亦显著高于肺良性病变肺组织 (P <0 .0 5 )。肺癌患者支气管切缘上皮增生组织阳性表达率 ( 4 0 .0 0 % )显著高于正常支气管上皮 ( 15 .15 % ) (P =0 .0 3 2 )。低分化肺癌hnRNPA2 /B1阳性表达率 ( 78.13 % )显著高于中 -高分化肺癌 ( 4 6.15 % ) (P <0 .0 5 ) ,伴有淋巴结转移肺癌阳性表达率 ( 75 .0 0 % )显著高于无淋巴结转移肺癌 ( 5 0 .0 0 % ) (P <0 .0 5 ) ,Ⅲ +Ⅳ期肺癌阳性表达率 ( 78.5 7% )显著高于Ⅰ +Ⅱ期 ( 5 0 .0 0 % ) (P <0 .0 5 ) ,T3 +T4肺癌阳性表达率 ( 77.42 % )显著高于T1+T2 ( 4 8.15 % ) (P<0 .0 5 )。不同组织学类型肺癌组织中hnRNPA2 /B1表达水平无显著性差异 (P >0 .0 5 )。结论 hnRNPA2 /B1的异常表达在肺癌的发生、发展和转移中?
Objective To investigate the expression features of the heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) in non-small cell lung cancer and its clinical significance. Methods hnRNP A2/B1 expression of cancer tissues, paracancerous lung tissues, resected bronchial stump epithelium tissues was detected in 58 non-small cell lung cancer patients and pulmonary tissues in 30 patients with benigh pulmonary lesions as control by immunohistochemistry methods. Results The positive expressive rate of hnRNP A2/B1 in lung cancer tissue (63.79%) was significantly higher than those in paracancerous lung tissue (43.10%) and benign pulmonary lesion tissues (20.00%) (P=0.000), and the positive rate in paracancerous lung tissue was also significantly higher than that in benign pulmonary lesion tissues (P<0.05). The positive rate of hnRNP A2/B1 in the hyperplastic and dysplastic epithelium (40.00%) of resected bronchial stump was remarkably higher than that in normal bronchial epithelium (15.15%) in lung cancer patients (P=0.032). The positive rate of hnRNP A2/B1 in poor differentiated lung cancer (78.13%) was remarkably higher than that in moderate and well differentiated lung cancer (46.15%) (P<0.05). The positive rate of hnRNP A2/B1 in lung cancer with lymph node metastasis (75.00%) was significantly higher than that without lymph node metastasis (50.00%) (P<0.05). The positive rate of hnRNP A2/B1 in stage Ⅲ+Ⅳ disease (78.57%) was significantly higher than that in stage Ⅰ+Ⅱ disease (50.00%) (P<0.05). The positive rate of hnRNP A2/B1 in T3+T4 cancer (77.42%) was significantly higher than that in T1+T2 cancer (48.15%) (P<0.05). The positive rate of hnRNP A2/B1 expression in lung cancer tissues was not related to histological classification of the cancer (P>0.05). Conclusion The overexpression of hnRNP A2/B1 in cancer tissues may play an important role in the oncogenesis, development and metastasis of lung cancer. Detection of hnRNP A2/B1 expression may be helpful to diagnose lung cancer and to predict prognos
出处
《中国肺癌杂志》
CAS
2004年第2期99-103,共5页
Chinese Journal of Lung Cancer
基金
国家"十五"攻关项目 (No.2 0 0 1BA70 3B1 1 )资助~~
