摘要
作者建立一个新的摹拟动脉壁结构的内皮和平滑肌细胞体外联合培养模型,并以此检测内皮、平滑肌细胞在合成和分泌组织型纤溶酶原激活物(t-PA)中的相互影响。结果表明,不同细胞组分的条件培养液(CM)中,t-PA活性有明显差异。内皮的CM中,t-PA活性最高,而平滑肌的CM中则最低。两种细胞联合培养时,培液的t-PA活性也明显比单纯内皮的降低。提示了细胞间的相互作用对血管壁溶栓机制的影响。
An in vitro endothelial (EC) and smooth muscle cell (SMC) cocultured model, which simulates the cell arrangement of the wall of artery in vivo, was established. The effect of interaction between EC and SMC on tissue-type plasminogen activator (t-PA) activity was studied by this model. The results showed that the t-PA activity in the conditioned medium (CM) of EC was the highest, while that in SMC was the lowest. When EC was cocultured with SMC, the t-PA activity in CM was lower than that in EC only (P<0.05). It suggested that the intercellular regulation may be very important in the mechanism of thrombolysis within blood vessel.
出处
《中山医科大学学报》
CSCD
1992年第4期18-22,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
"七五"国家医学科技攻关项目
国家自然科学基金
关键词
内皮肌
细胞培养
T-PA
细胞间作用
endothelium
muscle, smooth
cells, cultured
tissue-type plasminogen activity (t-pA activity)
intercellular effect
