摘要
以大白鼠下腹部岛状皮瓣为模型,针对氧自由基可引起岛状皮瓣缺血再灌流损伤的现象,以目前公认有效的氧自由基清除剂超氧化物歧化酶(SOD)为对照,观察丹参注射液预防缺血再灌流损伤的效果.通过超微结构和皮瓣存活的观察,证实岛状皮瓣存在缺血再灌流损伤和丹参注射液对缺血性岛状皮瓣具有保护作用。
Oxgyen-derived free radicals are important mediators of tissue injury in expermental island skin flaps that have been subjected to prolonzed ischemia(vascular occlusion)follow- ed by reperfusion.In this study,the role of oxgyen free radical scavenger,SOD,and a herb,salvia miltiorrhiza,in the protection of cellular damages during total ischemia and reperfusion was study in the epigastrie island skin flaps in experimental rats with electron microscopy and the assessment of survival of the flaps.Control flaps subjected to 10 hours of total vascular occlusion showed a high ineidenee of necrosis when followed for 7 days following release of the vascular occlusion.Treatment with superoxide dismutase and salvia miltiorrhiza prior to the onset of reperfusion significantly enhanced island flap sur- vival to 72.5%(P<0.001) and to 64.2%(P<0.05),respectively. The conclusions are: 1.Reperfusion for 10 hours following isehemia for 8 hours in the epigastrici sland flaps of the rats greatly exaggerated the original injury. 2.SOD and salvia miltiorrhiza may protect the flaps from such injury considerably and chanced flap survival.
