摘要
目的 通过检测增殖细胞核抗原在胃肠粘膜相关淋巴瘤 (简称MALT 淋巴瘤 )中的表达 ,探讨它在肿瘤发生、发展及预后判断中的意义。方法 利用免疫组化 (LSAB)方法对 6 0例胃肠MALT 淋巴瘤进行CD系列LCA、CD4 5RO、CD4 5RA、CD2 0、CD4 3标记 ,再次确定肿瘤细胞的组织学及免疫表型。利用免疫组化 (LSAB)方法检测增殖细胞核抗原 (PCNA)在 6 0例胃肠MALT 淋巴瘤中的表达。结果 6 0例均为MALT 淋巴瘤 ,其中 55例为B细胞型 ,5例为T细胞型 ,6 0例中低度恶性为 4 1例 ,高度恶性为 19例。该组病例中PCNA阳性检出 6 0例 ,阳性检出率为 10 0 % ,PCNA阳性信号位于肿瘤细胞核内。虽然所有病例中PCNA都呈阳性表达 ,但表达强度却不相同。 4 1例低度恶性中 34例表达强度为 +～ ,4例为 ,3例为 。 19例高度恶性中 3例为 ,6例为 ,10例为 。结果显示 :PCNA的表达强度随着肿瘤恶性程度的增强而增强 ,PCNA的表达程度与肿瘤的恶性程度有关。结论 增殖在肿瘤的发生、发展、转化中起着重要作用 ,检测增殖细胞核抗原可能是诊断恶性淋巴瘤、评价其生物学行为的可靠指标 。
Objective To investigate the significance of the ex pr ession of proliferating cell nuclear antigen (PCNA) in mucosa-related lymphoid tissue (MALT) lymphoma in the genesis, progression, and prognosis of MALT lympho ma. Methods LCA, CD45RO, CD45RA, CD20, and CD43 in 60 cases of MALT lymphoma were labeled by immunohistochemistry (labelled streptavidin biotin ,LSAB) to further confirm the histological types and immune phenotypes. PCNA exp ression in 60 cases of MALT lymphoma was detected by immunohistochemistry (LSAB) . Results Of the 60 cases of MALT lymphoma, B-cell lymphoma an d T-cell lymphoma were found in 55 and 5 cases, respectively. Low to middle -grade malignancy and high-grade malignancy were found in 41 and 19 cases, res pectively. PCNA expression was detected in all 60 cases (detection rate: 100%). PCNA positive signal was found in the nuclei of tumor cells. However, expression intensities of +～, , and were found in 34, 4, and 3 out of 41 cases of low-grade malignancy, respectively; expression intensities of , , and w ere found in 3, 6, and 10 out of 19 cases of high-grade malignancy. Conclusions The expression intensity of PCNA is associated with the grade of malignancy of MALT lymphoma. Proliferation plays an important role in the genesis, progression and transformation of MALT lymphoma. PCNA may be th e reliable index for the diagnosis of malignant lymphoma and the evaluation of t he biological behaviors of malignant lymphoma. Detection of PCNA expression may be beneficial for the prognosis of MALT lymphoma.
出处
《消化外科》
CSCD
2004年第2期125-127,共3页
Journal of Digestive Surgery
关键词
淋巴瘤
粘膜相关淋巴样淋巴组织
增殖细胞核抗原
免疫组化
lymphoma mucosa-related lymphoid tissue proliferating cell nuclear antigenimmunohistochemistry
