摘要
目的 :研究中药功劳木 (gonglaomu ,GLM)提取物在体外对白血病耐药细胞K5 6 2 /ADM和乳腺癌耐药细胞MCF7/ADM逆转MDR1的作用 ,以便寻找和确定逆转肿瘤细胞多药耐药有效的中药新药 .方法 :应用MTT法、台盼蓝拒染试验法测定应用GLM提取物后ADM对K5 6 2 /ADM ,MCF7/ADM细胞的细胞毒作用 ,应用Rh1 2 3荧光技术检测应用GLM提取物后对耐药细胞内药物浓度的影响 .结果 :应用GLM提取物后MTT法显示ADM对K5 6 2 /ADM ,MCF7/ADM细胞的抑制率高于未用GLM组 ,且有显著性差异 (P <0 .0 1 ) .台盼蓝拒染试验测定显示应用GLM提取物后ADM对K5 6 2 /ADM ,MCF7/ADM活细胞率为 34.5 %和 38.5 % ,低于未用GLM组的 6 8.2 %和77.4 % ,且有显著性差异 (P <0 .0 1 ) .逆转倍数K5 6 2 /ADM ,MCF7/ADM细胞分别为 6 .2 8和 5 .78.Rh1 2 3荧光技术显示随着GLM提取物浓度的提高K5 6 2 /ADM细胞内药物浓度随之增高 .结论 :GLM提取物可提高了ADM对耐药细胞的细胞毒作用 ,增加了耐药细胞内药物浓度 。
AIM: To investigate the effect of Chinese drug GLM (Gonglaomu) initial solution on K562/ADM leukemia resistant cell and MCF7/ADM breast cancer resistant cell line, in order to search new effective agents reversing MDR of tumor cell. METHODS: MTT (Mehyl thazol tetrazolinum) assay and trypan blue anti staining test were used to determine the cytotoxic effect of ADM, when combined with GLM, on K562/ADM cell line and MCF7/ADM cell line. Rhodamin 123 fluorescent dye retention test was used to assess the effect of GLM on intracellular concentration. RESULTS: MTT assay showed that the inhibition rate of K562/ADM and MCF7/ADM cell in GLM used group was higher than that in GLM non used group ( P <0.01). Trypan blue assay showed that the survival rate of K562/ADM, MCF7/ADM in GLM used group (34.5% and 38.5%) was lower than that in GLM non used group (68.2% and 77.4%, P <0.01). Reverse multiple of K562/ADM, MCF7/ADM cell was 6.28 and 5.78 respectively. Rh123 retention test suggested that GLM in combination with ADM could increase the intracellular ADM concentration. CONCLUSION: GLM could enhance the cytotoxic effect of ADM on drug resistant cell and increase intracellular drug concentration in a concentration dependent fashion.
出处
《第四军医大学学报》
CAS
北大核心
2004年第3期236-239,共4页
Journal of the Fourth Military Medical University
基金
吉林省科委重点课题 (2 0 0 0 0 30 3)
关键词
多药耐药
逆转
白血病
乳腺癌
肿瘤细胞
中药治疗
multi drug resistance
reversal
leukemia
breast neoplasm
tumor cells
Chinese drug treatment
