摘要
目的 比较表皮生长因子 (EGF)、促甲状腺素释放激素 (TRH)及地塞米松 (Dex)对胎兔肺表面活性物质蛋白 (SP) A、SP B、SP CmRNAs表达的影响。方法 64只妊娠兔在妊娠第 2 2天或 2 4天 ,分别静脉注射Dex(Dex组 )、EGF(EGF组 )、TRH(TRH组 )和生理盐水 (对照组 ) ,每组 16只。于妊娠第 2 5天及第 2 7天处死妊娠兔取胎肺。采用逆转录 聚合酶链反应 (RT PCR)和DNA印迹法检测胎肺SP A、SP B及SP C的mRNAs水平。结果 ( 1)对照组、EGF组、TRH组及Dex组在妊娠第 2 7天时 ,胎肺SP BmRNA的相对值分别为 0 67± 0 14、1 0 1± 0 3 3、1 17± 0 48、1 0 4± 0 2 7,EGF组、TRH组及Dex组均显著高于对照组 (P <0 0 5) ;在妊娠第 2 5天 ,SP A和SP CmRNAs在各组胎肺均有较高水平表达 ,但SP BmRNA仅能在Dex组检出 ,相对值为 0 58。 ( 2 )在妊娠第 2 7天 ,胎肺中的SP BmRNA水平 ( 0 67)明显低于SP AmRNA( 1 2 1)和SP CmRNA( 1 2 6) (P <0 0 1) ;SP A和SP CmRNAs在各组间比较 ,差异无显著性 (P <0 0 5)。结论 ( 1)胎兔肺SP A和SP CmRNAs表达早于SP BmRNA ,而相同样本的SP BmRNA表达水平低于SP A和SP CmRNAs ;( 2 )Dex、EGF和TRH均可促进胎兔肺SP BmRNA的表达 ,但在妊娠的早期 。
Objective To compare the effect of epidermal growth factor (EGF), thyrotropin releasing hormone (TRH) and dexamethasone (Dex) on the expression of surfactant protein (SP) A, SP B and SP C mRNA in fetal rabbit lungs Methods Sixty four Japanese white pregnant rabbits, put into four different groups(16 rabbits each), were given EGF(EGF group), TRH(TRH group), Dex(Dex group) or normal saline(control group)intravenously from gestational day 22 nd (5 rabbits in each group) or 24 th (11 rabbits in each group) for three days before killing The levels of SP A, SP B and SP C mRNAs in the fetal rabbit lungs were detected by reverse transcription polymerase chain reaction (RT PCR) and Southern blotting Results In the 27 th gestational day ,SP B mRNA levels in the fetal rabbit lungs in all treated groups were found significantly higher than those in the control group( P <0 05) In the 25 th day ,there had a higher expression of SP A and SP C mRNAs in the fetal lungs in all groups,but SP B mRNA could be tested only in the Dex group SP B mRNA level was lower than that of SP A mRNA and SP C mRNA in the same fetal lungs in the 27 th gestationed day Conclusion The time of detectable SP B mRNA in rabbit fetal lungs appeared later than that of the SP A and SP C mRNAs The expression of SP B mRNA is lower in comparison with those of SP A mRNA and SP C mRNA in the same samples Prenatal maternal treatment with intravenously Dex, EGF and TRH could increase the expression of SP B mRNA in fetal rabbit lungs, and the effect of Dex may be more significant at earlier stage of gestation
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2003年第9期541-544,共4页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金资助项目 ( 3970 0 1 49)
