摘要
目的 通过观察肺组织中的二棕榈酰磷脂酰胆碱 (dipalmitoylphosphatidylcholine ,DPPC)含量的变化 ,反映肺表面活性物质 (pulmonarysurfactant,PS)的含量与小鼠对全氟异丁烯 (perfluoroisobutylene ,PFIB)中毒耐受性的关系 ,以揭示小鼠对PFIB中毒耐受的机制。方法 采用静式全暴露法 ,分别以 710、775和 12 5 0 μg (L·min) 浓度的PFIB 3次染毒小鼠。用干湿重法测定每次中毒后活存小鼠的肺水含量 ,并采用高效液相色谱法 (HPLC)测定其肺组织磷脂中DPPC的含量。结果 (1)第 2次中毒和第 3次中毒小鼠的活存率均明显高于其对照组 (P <0 0 1) ;(2 )耐受PFIB小鼠的肺水含量与正常对照组相比没有差别 ;(3 )中毒耐受小鼠的肺组织中DPPC含量均明显高于对照组 (P <0 0 5或P <0 0 1)。结论 急性低浓度PFIB重复中毒的小鼠在致死剂量PFIB染毒时没有出现死亡 ,其肺组织中DPPC的含量较正常对照组显著增高 。
Objective To investigate the relationship between the content of pulmonary surfactant(PS) and the tolerance of mice inhaled perfluoroisobutylene(PFIB) and to reveal the possible mechanisms of tolerance produced by PFIB in mice. Methods One hundred Kunming mice were used and divided into two groups randomly:one as the control and the other as the experiment. PFIB was inhaled at the doses of 710,775, 1 250 μg/L·min each after each. Vital mice were killed to take its lungs when 144 hours after the first poisoning and 96 hours after the second poisoning and 96 hours after the third poisoning. The right lung was used for measurement of water content,while the left lung was determined the level of dipalmitoylphosphatidylcholine(DPPC) by high performance liquid chromatography(HPLC).Results 1.The survival rates of the experimental mice were higher than that of the control group( P <0 01). 2.The content of lung water were not different between the experimental and the control group. 3.The levels of DPPC in the tolerant mice were markedly increased compared with those in the control( P<0 05). Conclusion Remarkable changes in PS can be responsible for the protection of acute lung injury following acute PFIB exposure. It gives a clue for prevention and treatment of pulmonary injury after acute PFIB exposure.
出处
《卫生毒理学杂志》
CSCD
北大核心
2004年第1期18-20,共3页
Journal of Health Toxicology
基金
全军"十五"指令性课题 (0 1L0 77)
