摘要
本文建立了高效液相色谱法测定人血浆和尿中头孢唑肟浓度。血样经高氯酸沉淀蛋白后,直接进样测定;尿样稀释后直接进样测定。固定相为C_(18),5μm颗粒,流动相为乙腈:水:磷酸:二乙胺—1:9:0.013:0.017(v/v)。检测波长为UV254nm(血浆)和290nm(尿),采用内标法定量。方法的线性范围分别为1～160μg/ml(血浆)和10～1600μg/ml(尿),检测限为2ng,平均回收率分别为99.0±1.0%(血浆)和100.3±0.6%(尿)。日内及日间CV%均小于5%。
A reversed phase HPLC method was established for analysis of ceftizoxime
concentrations in plasma and urinc. The optimal assay procedures were set up
after a series of studies were done on the pH of mobile phase, the proportion of
acetonitrile in mobile phase, and the selection of internal standard and preci-
pitant for plasma, pretreatment. 6% of perchloric acid was used to remove the pl-
asma protein and an aliquot of supernatant was injected into HPLC. For assaying
ceftizoxime in urine, the samples was diluted 10 times with water before injected
directly. The analytical column (150×5mm) was packed with C_(18), particle size 5
μm. Mobile phase consisted of acetonitrile: water: phosphoric: diethylamine-1:9
: 0.013: 0.017(v:v), pH=3). Flow rate was 1 ml/min and ceftizoxime was detected
at UV 254 nm for plasma and 290 nm for urine. Acetazolamide was used as a inte-
rnal standard. The methed was linear within 1～160 μg/ml for plasma and 10～1600
μg/ml for urine with the relative coefficient from 0.9996～0.9999 for plasma and u-
rine. The detective limit was found to be 2ng. The recoveries averaged 99.0±1.0%
and 100.3±0.6% for plasma and urine respectively, with CV less than 5% both
for within and between days.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1992年第4期149-151,共3页
Chinese Journal of Hospital Pharmacy
关键词
头孢唑肟
高效液相色谱
ceftizoxime
plasma drug concentration
urine drug concentration
HPLC
