摘要
目的 探讨大鼠岛状皮瓣缺血 再灌注损伤中性粒细胞数量的动态变化 ,阐明中性粒细胞黏附血管壁及组织浸润是这一变化的重要原因。方法 36只大鼠等分为 3组。皮瓣原位缝合组 (Ⅰ组 ) ;实验组 (Ⅱ组 ) :再灌注时腹腔注射地塞米松 5mg/kg ;对照组 (Ⅲ组 ) :再灌注时腹腔注射生理盐水2ml/kg。 0、1、3、5、7d时分析皮瓣蒂部血液及皮瓣中性粒细胞变化规律 ,检测皮瓣蒂部及耳部中性粒细胞黏附功能变化。结果 蒂部血液中性粒细胞变化规律 :Ⅰ组比较恒定 ,Ⅱ、Ⅲ组缺血 8h时上升 ,再灌注1d时II组明显大于Ⅲ组 ,3d时Ⅱ组呈明显下降趋势 ,5d时恢复正常 ,Ⅲ组 5d后中性粒细胞呈现上升趋势。皮瓣中性粒细胞浸润数目 :1、3d时Ⅲ组明显大于Ⅱ、Ⅰ组 ;蒂部粒细胞黏附数量 :再灌注开始时Ⅲ、Ⅱ组 >Ⅰ组 ,1、3、5、7d时Ⅲ组 >Ⅱ、Ⅰ组。耳部中性粒细胞黏附数量与蒂部呈现相同的变化规律。结论 中性粒细胞黏附血管壁及组织浸润是岛状皮瓣缺血 再灌注损伤的重要原因 ,也是 3组间中性粒细胞呈现不同变化特点的重要原因。
Objective To study neutrophil dynamic changes in ischemic-reperfusion injury flap, to observe neutrophil adhesion with endothelial cell and neutrophil sequestration in tissue, and to elucidate the important role of neutrophil in ischemic-reperfusion injury flap. Methods A total of 36 rats were divided into sham group ( group Ⅰ), dexamethasone treatment group ( group Ⅱ), and saline control group (group Ⅲ). Neutrophil dynamics and neutrophil adhesion function on 0, 1, 3, 5,and 7 days were analysed. Results Neutrophil number in pedicle venous blood showed constant in group I, and increased in group II, more than that in group Ⅲ on day 1, decreased on day 3 and reached normal level on day 5 in group Ⅱ; it increased on 5 days in group Ⅲ. Neutrophil sequestration numbers in flap were higher in group Ⅲ than that in group I and II on 1, 3 days. Neutrophil adhesion numbers and change pattern in pedicle and ear were the same. Conclusion Neutrophils adhesion with endothelial cells and sequestration in tissues is the important reason of injury in ischemia- reperfusion flap and it is also the reason why different changes of neutrophil occur in different groups.
出处
《中华医学美学美容杂志》
2003年第3期169-171,共3页
Chinese Journal of Medical Aesthetics and Cosmetology
